葡萄籽与青蒿提取物对黄鳝肠道消化酶活性及血液生化指标的影响

选择尾均体质量为25g的健康黄鳝（Monopterusalbus）2400尾，分为5组，1个对照组投喂基础饲料，4个试验组分别在基础饲料中添加10g·kg-1、20g·kg-1、30g·kg。和40g·kg-1葡萄（Vitisamurensis）籽与青蒿（Arte—misiaannua）提取物（质量比为4：1）的混合物，测定了在2l～28℃水温下饲养100d后鱼体肠道消化酶活性及血液生化指标。结果显示，基础饲料中添加植物提取物能提高鱼体肠道消化酶、血液谷草转氨酶（GOT）与谷丙转氨酶（GPT）的活性以及血清总蛋白（TP）的含量，降低血液葡萄糖（GLU）和甘油三酯（TG）的水平。与对照组相比，添加量为20g·kg-1和30g·kg-1时上述指标均有显著差异（P〈0．05）；添加量为10g·kg-1和40g·kg-1时差异不显著（P〉0．05）。20g·kg-1与30g·kg-1组间各项指标无显著差异（P〉0．05）。试验表明，葡萄籽和青蒿提取物作为饲料添加剂添加量为20～30g·kg-1时能显著提高黄鳝肠道消化酶的活性、调节鱼体血液生化指标，具有促进消化和三大营养物质代谢活动、加快鱼体生长的作用。

Effects of plant extract from grape seed and sweet wormwood on intestinal digestive enzyme activities and blood biochemical parameters of eel (Monopterus albus)

To explore the growth promotion mechanism of plant extract from grape （ Vitis amurensis ） seed and sweet wormwood on Monopterus albus, 2 400 healthy eel fingerlings were randomly divided into five groups. We fed control group with basal feed, and four experimental groups with diets containing respectively 10 g.kg-I , 20 g.kg-l , 30 g.kg -1, 40 g.kg-1 mixture, which were ex- tracted from grape seed and from sweet wormwood （Artemisia annua） with a four-to-one proportion. We determined the digestive en- zyme activities and blood biochemical parameters of five eel groups after feeding for 100 d in 21 - 28~C. Results show that basal diets with the mixture could improve digestive enzyme, blood glutamic-oxaloacetic transaminase （GOT） and glutamic-pyruvic transami- nase （GPT）＇s activities, and total protein （TP）＇s content; and could reduce blood glucose （GLU） and triglyceride （TG）＇s con- tents of eel. Compared with the control group, the above indexes had significant difference （P 〈 0.05） when the dosage of the plant extract was 20 g·kg-1 or 30 g·kg-1, while the above indexes had no significant difference （P 〉 0. 05 ） when it was 10 g.kg-1or 40 g·kg-1. There was no significant difference between 20 g·kg-l and 30 g·kg 1 experimental groups. In conclusion, extract from grapeseed and sweet wormwood with a four-to-one ratio, when mixed in diets with dosage 20 -30 g-kg-1, could increase digestive en- zyme activities, regulate blood biochemical parameters, enhance the digestion and metabolic activity of 3 main nutritive materials and improve the growth of fish.