| 超高效液相色谱-串联质谱法测定凡纳滨对虾中的硝基呋喃代谢物残留 |
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| 引用本文： | 赵艳,张凤枰,刘耀敏.超高效液相色谱-串联质谱法测定凡纳滨对虾中的硝基呋喃代谢物残留[J].南方水产,2011,7(4):55-60. |
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| 作者姓名： | 赵艳 张凤枰 刘耀敏 |
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| 作者单位： | 通威股份有限公司,四川成都,610041 |
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| 摘 要： | 采用超高效液相色谱-串联质谱（UPLC-MS/MS）法,测定了凡纳滨对虾（Litopenaeus vannam ei）中氨基脲（SEM）、1-氨基-2-内酰脲（AHD）、3-氨基-2-唑烷基酮（AOZ）和5-甲基吗啉-3-氨基-2-唑烷基酮（AMOZ）4种硝基呋喃类代谢物。采用2-硝基苯甲醛作为衍生化试剂,氘、碳-13、氮-15同位素标记的SEM1-3C1-5N2、AHD1-3C3、AOZ-D4和AMOZ-D5为内标物,样品经衍生、乙酸乙酯提取、正己烷净化,以甲醇0.5 mmol.L-1乙酸铵水溶液（含0.1%甲酸）为流动相进行梯度洗脱,8 m in可将4种目标化合物完全分离并测定。结果表明,4种待测物在0.4～20.0 ng.mL-1范围内线性关系良好（R〉0.999）,检出限为0.03～0.15μg.kg-1。在0.5μg.kg-1、1.0μg.kg-1和2.0μg.kg-13个水平上进行加标回收试验,平均回收率为92.0%～107.3%,RSD为3.1%～8.7%。采用该法参加FAPAS国际水平测试,获得了满意的结果。
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| 关 键 词： | 硝基呋喃类代谢物 残留 凡纳滨对虾 超高效液相色谱-串联质谱法 |
Determination of residues of nitrofuran metabolites in Litopenaeus vannamei by UPLC-MS/MS |
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| ZHAO Yan,ZHANG Fengping,LIU Yaomin.Determination of residues of nitrofuran metabolites in Litopenaeus vannamei by UPLC-MS/MS[J].South China Fisheries Science,2011,7(4):55-60. |
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| Authors: | ZHAO Yan ZHANG Fengping LIU Yaomin |
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| Institution: | ZHAO Yan,ZHANG Fengping,LIU Yaomin(Tongwei Co.,Ltd.,Chengdu 610041,China) |
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| Abstract: | Four nitrofuran metabolites,including semicarbazide(SEM),1-amino-hydantoin(AHD),3-amino-2-oxazolidinone(AOZ) and 5-methylmorpholino-3-amino-2-oxazolidinone(AMOZ) in Litopenaeus vannamei,were determined by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).SEM-13C-15N2,AHD-13C3,AOZ-D4 and AMOZ-D5 were selected as internal standard substances;the samples were derived by 2-nitrobenzaldehyde,extracted with ethyl acetate and cleaned up with n-hexane.Four targeted compounds were complete... |
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| Keywords: | nitrofuran metabolites residues Litopenaeus vannamei UPLC-MS/MS |
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