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| 理化因子对杂色鲍血蓝蛋白酚氧化酶活性影响的研究 | |
| 引用本文： | 彭文,姜敬哲,王江勇,丁雪娟.理化因子对杂色鲍血蓝蛋白酚氧化酶活性影响的研究[J].南方水产,2010,6(2):1-6. |
| 作者姓名： | 彭文 姜敬哲 王江勇 丁雪娟 |
| 作者单位： | 1. 中国水产科学研究院南海水产研究所,广东,广州,510300;华南师范大学生命科学学院,广东,广州,510631 2. 中国水产科学研究院南海水产研究所,广东,广州,510300 3. 华南师范大学生命科学学院,广东,广州,510631 |
| 基金项目： | 现代农业产业技术体系建设专项资金资助项目(nycytx-47);;广东省科技推广项目(2006B40101003);;广东省重大科技兴海项目(A200701D01);;中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院南海水产研究所)资助项目(2007ZD09) |
| 摘 要： | 以邻苯二酚为底物研究部分理化因子对杂色鲍（Haliotis diversicolor）血蓝蛋白酚氧化酶活性的影响。结果表明，血蓝蛋白酚氧化酶活性最适温度34℃、最适pH6．4；铜离子（Cu^2＋）能极其显著增强血蓝蛋白的酚氧化酶活性，增强率为264％，而乙二胺四乙酸二钠（EDTA）、镁离子（Mg^2＋）和锌离子（Zn^2＋）则能极其显著地抑制其活性，EDTA的抑制效率最高，抑制率为52．8％，Mg^2＋和Zn^2＋的抑制率分别为27．8％和25％。钙离子（Ca^2＋）对血蓝蛋白酚氧化酶活性影响不显著。 |
| 关 键 词： | 理化因子 杂色鲍 血蓝蛋白 酚氧化酶活性 |
Effect of physicochemical factors on phenoloxidase activity of hemocyanin from abalone Haliotis diversicolor |
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| PENG Wen,JIANG Jingzhe,WANG Jiangyong,DING Xuejuan.Effect of physicochemical factors on phenoloxidase activity of hemocyanin from abalone Haliotis diversicolor[J].South China Fisheries Science,2010,6(2):1-6. | |
| Authors: | PENG Wen JIANG Jingzhe WANG Jiangyong DING Xuejuan |
| Institution: | 1. South China Sea Fisheries Research Institute/a>;Chinese Academy of Fishery Sciences/a>;Guangzhou 510300/a>;China/a>;2. Life Science College/a>;South China Normal University/a>;Guangzhou 510631/a>;China |
| Abstract: | The effect of physicochemical factors on phenoloxidase activity of hemocyanin from Haliotis diversicolor was studied with catechol as substrate. The results showed that the optimum temperature for enzyme activity was 34℃ and the optimum pH was 6.4, respectively. Cu^2＋ could significantly activate enzyme activity and the enhancement rate was 264%. However, EDTA, Mg^2＋ and Zn^2＋ could significantly inhibit enzyme activity. The inhibition rate was 52. 8%, 27.8% and 25%, respectively, and EDTA had the highest rate. Ca^2＋ had no significant effect on enzyme activity. |
| Keywords: | physicochemical factors abalone （Haliotis diversicolor） hemocyanin phenoloxidase activity |
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