间歇投喂添加蛋氨酸硒饲料对仿刺参（Apostichopus japonicus Selenka）幼参生长和免疫指标的影响

在水温9℃～13℃下,将平均体质量0.745±0.3 g的仿刺参（Apostichopus japonicus Selenka）随机投放到50cm×40cm×30cm的塑料水槽内,每箱10头,共分为五组,每组3个平行,采用不同间隔时间投喂每kg添加0.5 mg蛋氨酸硒的饲料：第1、2、3、4组分别间隔0、5、10和15...

Effects of alternatively feeding regimes of dietary selenomethionine on growth and some immune indices in juvenile sea cucumber(Apostichopus japonicus)

The effects of feeding regimes on growth and immune indices in juvenile sea cucumber（Apostichopus japonicus） with body weight of 0.745±0.3g fed the diet containing selenomethionine（0.5 mg/kg） were studied at water temperature ranging from 9℃ to 13℃ from March 10,2010,to May 9,2010 in a lab.Total of 150 individuals were selected,randomly stocked into tanks of each 50 cm ×40 cm ×30 cm at a rate of 10 individuals per tank,and fed the diet containing seleniomethionine once daily（Group 1）,once every 5 days（Group 2）,once every 10 days（Group 3）,once every 15 day（Group 4） and once daily for the diet without selenium（Group 5,as control group） with triplication for 60 days.The results showed that the sea cucumber fed the diets containing selenium had significantly higher survival than that in the control group（P 0.05）,even though they had no significant differences in the specific growth compared with the animals in the control group（P 0.05）.There were very significantly higher activities of superoxide dismutase（SOD）（78.34 U/ml） and glutathione（GSH）（2.87×10-5mol/L） in the Group 2 than those in the control group（P 0.05）.No significant difference in the concentration of GSH was found between Group 2 and Group 3,and Group 4（P 0.05）.Compared with the other groups,there were higher acid and alkaline phosphatase activities（2.42 U/ml,and 5.76 U/ml） in Group 1,without significant difference（P 0.05）.It is recommended that the sea cucumber be fed the diet containing seleniumomethionine every day for promotion of survival and immunity.