“红肉病”文蛤中寄生类立克次体的超微结构与细胞病理学

报道了患“红肉病”文蛤的消化盲囊上皮中所发现的类立克次体以及所引起的受感染细胞的病理学变化。该病原包涵体呈球形或椭球形,有强嗜碱性和嗜酸性两种,电镜下观察到上皮细胞内充满了大量的类立克次体,其形态结构及大小表现了极大的多样性,受感染的细胞具有明显的病理学变化,内质网膨大为潴泡状,核糖体脱落;线粒体溶解或固缩,核膜肿胀,染色质固缩并出现液泡结构,高度感染时,细胞核与大部分的细胞器消失,在上皮细胞中,观察到类立克次体以二分裂和出芽方式进行繁殖。

On ultrastructure and cytopathology of the rickettsia-like organisms parasitized in the Meretrix meretrix Linnaeus suffering from

From June to July 2000,specimens of Meretrix meretrix Linnaeus which suffered from the "red muscle disease"(RMD)were collected from Qinhuangdao district of Hebei province.The rickettsia like organisms (RLO)were found and observed in the digestive diverticula. There are two types of RLO inclusion bodies,one is basophilia and another is acidophilia,both of which observed are circularity or olivary shapes.By the electron microscope,the epithelial cells were filled with RLO,of which the shapes and sizes are different.The infected cells with RLO presented a series obvious pathological changes.The endoplasmic reticulum swollen and mostly turned into vesicles,the ribosomes degenerated.The mitochondria dissolved or condensed.The nucleus underwent a series of changes including the chromatin condensing,nuclear membrane swelling and forming nuclear vesicles. At last,the nucleus and most of cellular organelles disappeared.The RLO in binary fission and budding in the cytoplasm were observed.