南美白对虾必需氨基酸的需要量

采用高生物价的酪蛋白、明胶为蛋白源的蛋白饲料(PD)和无蛋白饲料(FPD)饲养南美白对虾幼虾(0.2627～0.2715g),根据虾体必需氨基酸(EAA)生长及维持量代谢,研究南美白对虾必需氨基酸的需求量。结果表明,要满足幼虾正常生长,各种EAA需求量g·(100g虾体重)-1·d-1]为苏氨酸(Thr)0.046,缬氨酸(Val)0.054,蛋氨酸(Met)0.029,异亮氨酸(Ile)0.069,亮氨酸(Leu)0.087,苯丙氨酸(Phe)0.051,赖氨酸(Lys)0.086,组氨酸(His)0.025,精氨酸(Arg)0.097。如饲料蛋白质水平为40%,蛋白质利用率50%,虾摄食率为20%,推算得出饲料中EAA需求量(g·kg-1):苏氨酸(Thr)11.5,缬氨酸(Val)13.5,蛋氨酸(Met)7.3,异亮氨酸(Ile)17.3,亮氨酸(Leu)21.8,苯丙氨酸(Phe)12.8,赖氨酸(Lys)21.5,组氨酸(His)6.3,精氨酸(Arg)24.3。

Requirement of essential amino acids for Penaeus vannamei

Feeding experiments were conducted with protein diets ( PD) and protein..free diets ( FPD) for juvenile Penaeus vannamei ( average body weight s, 0. 2627- 0. 2715g ) . The diets( PD) are prepared by casein and gelat in as protein source with high biological values. The requirements of juvenile Penaeus vannamei for essential amino acids ( EAA) were determined based on the daily depo sition and changes of each EAA in shrimp body. The minimum requirements of EAA needed to satisfy the requirements of juvenile Penaeus vannamei growth ( g- 1..( 100g body weight) - 1..d- 1) were Threonine 0. 046, Valine 0. 054, Methionine 0. 029, Isoleuline 0. 069; Leucine 0. 087, Phenylalanine 0. 051, Lysine 0. 086, Histidine 0. 025, Arginine 0. 097. When dietary protein levels was 40%, net protein ut ilization ratio was 50% and the feeding rate was 20% of the body weight, the minimum contents of EAA in the dietary ( g ..kg - 1 diet ) were as follow s, Threonine 11. 5, Valine 13. 5, Methionine 7. 3, Isoleuline 17. 3, Leucine 21. 8; Phenylalanine 12. 8, Lysine 21. 5, Histidine 6. 3 and Arginine 24. 3.