| 发酵豆粕替代鱼粉对凡纳滨对虾生长、免疫相关酶及免疫相关基因表达的影响 |
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| 引用本文： | 刘韬,黄旭雄,苏美英,杨景丰,郭子好,孔纯,华雪铭.发酵豆粕替代鱼粉对凡纳滨对虾生长、免疫相关酶及免疫相关基因表达的影响[J].水产学报,2018,42(9):1417-1427. |
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| 作者姓名： | 刘韬 黄旭雄 苏美英 杨景丰 郭子好 孔纯 华雪铭 |
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| 作者单位： | 农业部淡水水产种质资源重点实验室上海海洋大学;水产科学国家级实验教学示范中心上海海洋大学;农业部鱼类营养与环境生态研究中心上海海洋大学;烟台南山学院工学院;上海源耀生物股份有限公司 |
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| 基金项目： | 上海市科委高校能力建设项目（14320502000） |
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| 摘 要： | 从免疫相关酶活及基因转录水平角度探讨发酵豆粕替代鱼粉对凡纳滨对虾健康生长及免疫机能机制的影响。实验设置5种实用饲料,以30%鱼粉组(FM)为对照组,分别用4%(FSM4)、8%(FSM8)、12%(FSM12)和16%(FSM16)的发酵豆粕,替代9.7%、19.4%、29.1%和38.8%鱼粉,分为4个处理组,饲养体质量为(7.62±0.23)g的凡纳滨对虾60 d后,统计生长性能,检测肌肉营养成分、血清及肝胰腺免疫相关酶活性,肝胰腺HSP70和鳃Toll受体、IMD、溶菌酶(LZM)免疫相关基因m RNA的表达水平。结果显示:(1)与对照组相比,发酵豆粕替代鱼粉对凡纳滨对虾成活率无显著影响;过低或过高水平的发酵豆粕替代鱼粉皆会影响凡纳滨对虾的特定生长率。(2)除FSM12组外,肌肉粗蛋白含量发酵豆粕替代组均低于对照组;粗脂肪含量随着发酵豆粕替代量的升高而降低,FSM16组最低。(3)血清谷丙转氨酶活FSM4和FSM16组显著高于对照组;谷草转氨酶活FSM4组最高,而FSM8组最低;除FSM12组外;碱性磷酸酶活性发酵豆粕替代组显著高于对照组;除FSM16组外,血清总蛋白与肝胰腺丙二醛含量发酵豆粕替代组与对照组无显著性差异。(4)随着发酵豆粕替代量增加,鳃Toll受体m RNA表达呈上升趋势,鳃IMD m RNA表达则呈先升后降趋势,发酵豆粕替代比例过高会降低鳃LZM m RNA表达水平,而肝胰腺HSP70 m RNA表达量则随着发酵豆粕替代比例增加呈上升趋势。综上所述,发酵豆粕适量替代鱼粉对凡纳滨对虾生长性能无显著影响,并可提高免疫相关酶活,改变免疫相关基因的表达。本实验条件下适宜发酵豆粕用量为8%~12%;替代量过高,会引起机体的过度应激。
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| 关 键 词： | 凡纳滨对虾 发酵豆粕 鱼粉 生长 免疫相关基因 |
| 收稿时间： | 2017/12/25 0:00:00 |
| 修稿时间： | 2018/4/6 0:00:00 |
Effects of fermented soybean meal replacing fish meal on the growth performance, immune-related enzymes and gene expression of Litopenaeus vannamei |
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| LIU Tao,HUANG Xuxiong,SU Meiying,YANG Jingfeng,GUO Zihao,KONG Chun and HUA Xueming.Effects of fermented soybean meal replacing fish meal on the growth performance, immune-related enzymes and gene expression of Litopenaeus vannamei[J].Journal of Fisheries of China,2018,42(9):1417-1427. |
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| Authors: | LIU Tao HUANG Xuxiong SU Meiying YANG Jingfeng GUO Zihao KONG Chun and HUA Xueming |
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| Institution: | Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,College of Engineering, Yantai Nanshan University, Yantai 265713, China,Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China,Shanghai Yuanyao Biotechnology Co. Ltd, Shanghai 201316, China,Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China and Centre for Research on Environmental Ecology and Fish Nutrition(CREEFN), Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China |
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| Abstract: | |
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| Keywords: | Litopenaeus vannamei fermented soybean meal fish meal growth immune-related genes |
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