| 卵黄抗体对凡纳滨对虾生长、免疫及抗VpAHPND感染的影响 |
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| 引用本文： | 王仁宝,史成银,宋晓玲,王一婷,张惠芬,任宁欣,谢国驷,张庆利.卵黄抗体对凡纳滨对虾生长、免疫及抗VpAHPND感染的影响[J].水产学报,2022,46(12):2409-2420. |
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| 作者姓名： | 王仁宝 史成银 宋晓玲 王一婷 张惠芬 任宁欣 谢国驷 张庆利 |
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| 作者单位： | 上海海洋大学水产与生命学院,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所,上海海洋大学水产与生命学院,中国水产科学研究院黄海水产研究所,中国水产科学研究院黄海水产研究所 |
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| 基金项目： | 农业农村部农业国际合作交流项目；国家自然科学基金 (31802342)；中国水产科学研究院基本科研业务费 (2020TD39) |
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| 摘 要： | 为探讨特异性卵黄抗体抗致急性肝胰腺坏死病副溶血弧菌 (Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease, VpAHPND) 感染的效果及其机制,防控对虾急性肝胰腺坏死病 (Acute hepatopancreatic necrosis disease, AHPND),本研究以添加不同剂量VpAHPND卵黄抗体制剂 (0、0.2%和0.5%) 的饲料投喂凡纳滨对虾幼虾,测定对虾的生长率和存活率、对虾肝胰腺免疫酶活力和免疫基因相对表达水平,通过浸浴感染实验测定免疫对虾抗VpAHPND感染的能力。生长实验结果显示,免疫28 d后,免疫组与未添加卵黄抗体制剂的对照组对虾在平均生长率、特定生长率和存活率方面均无显著性差异。免疫功能实验结果显示,免疫14 d后,与对照组相比,0.2%免疫组对虾肝胰腺的酚氧化酶 (PO)、超氧化物歧化酶 (SOD)、溶菌酶 (LZM) 活力显著升高,抗菌肽 (Crustin) 基因的相对表达水平也显著升高,而β-1,3-葡聚糖结合蛋白-脂蛋白 (β-GBP-HDL) 基因的相对表达水平则显著降低。浸浴感染实验结果显示,0.2%免疫组对虾的存活率显著高于对照组；0.2% VpAHPND卵黄抗体制剂对凡纳滨对虾的相对免疫保护率为63.77%。研究表明,口服卵黄抗体不会对凡纳滨对虾的生长和存活产生不良影响,0.2%的VpAHPND卵黄抗体制剂通过提升凡纳滨对虾的PO、SOD、LZM活力和Crustin基因表达水平,增强凡纳滨对虾的免疫功能,从而提高对虾抗VpAHPND感染的能力,具有很强的应用潜力。本研究为使用特异性卵黄抗体防控AHPND提供了依据,也为其作用机制研究提供了参考。
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| 关 键 词： | 卵黄抗体 凡纳滨对虾 生长性能 急性肝胰腺坏死病 免疫应答 基因表达 |
| 收稿时间： | 2021/2/3 0:00:00 |
| 修稿时间： | 2021/3/12 0:00:00 |
Effect of IgY on the growth, immunity and resistance to VpAHPND infection of Litopenaeus vannamei |
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| WANG Renbao,SHI Chengyin,SONG Xiaoling,WANG Yiting,ZHANG Huifen,REN Ningxin,XIE Guosi,ZHANG Qingli.Effect of IgY on the growth, immunity and resistance to VpAHPND infection of Litopenaeus vannamei[J].Journal of Fisheries of China,2022,46(12):2409-2420. |
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| Authors: | WANG Renbao SHI Chengyin SONG Xiaoling WANG Yiting ZHANG Huifen REN Ningxin XIE Guosi ZHANG Qingli |
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| Institution: | College of Fisheries and Life Science,Shanghai Ocean University,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,College of Fisheries and Life Science,Shanghai Ocean University,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Key Laboratory of Maricultural Organism Disease Control,Ministry of Agriculture and Rural Affairs,Laboratory for Marine Fisheries Science and Food Production Processes,Pilot National Laboratory for Marine Science and Technology Qingdao,Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity |
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| Abstract: | |
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| Keywords: | Immunoglobulin of yolk (IgY) Litopenaeus vannamei growth performance immune response gene expression acute hepatopancreatic necrosis disease (AHPND) |
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