猪圆环病毒2型分离株的基因组克隆、序列测定与分析

设计合成两对覆盖PCV2基因组奎长的引物，对PCV2分离株S2、P11、L进行全基因测序。将所测序列与GenBank上已公布的PCV2毒株序列进行同泺性比较，并绘制系统发育进化树。结果显示。所测毒株与其它地城的24株PCV2分离株的基因组序列同源性很高。3个分离株之问的奎基因核苷酸同源性在95.4％-99.3％。序列分析表明欧洲分离株和美洲分离株分别构成PCV2的两个夫的分支：欧洲系和美洲系。S2、P11属于欧洲系，L属于美洲系。

The Molecular Cloned,Sequencing and Analysis of Genome of Pordne circovirus 2

3 strains of PCV2 were isolated from the affected pigs suffered with PMWS. The full-length genomes of three isolates are amplified with two pairs of primers and cloned. The whole genomes are sequenced and compared. The sequences of three isolates were submitted to GenBank. Genome comparisons show significant differences between PCV1 and PCV2. Phylogenetie analysis of 27 strains reveals overall nueleotide sequence homology of PCV2 is highly, and diversity of different geographic distribution not significant. As showed by the phylagenetie relationships, PCV2 has two large apparent branches; European series and Amereian series. S2,P11 belong to European series,L belongs to Amercian series.