| 雌核发育鲢RAPD指纹和蛋白质电泳研究 |
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| 引用本文： | 邓怀,张四明,汪登强,潘光碧.雌核发育鲢RAPD指纹和蛋白质电泳研究[J].淡水渔业,1998,28(6):10-13. |
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| 作者姓名： | 邓怀 张四明 汪登强 潘光碧 |
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| 作者单位： | 中国水产科学研究院长江水产研究所,湖北荆州,434000 |
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| 摘 要： | 用２３个随机引物对雌核发育鲢子一代（普通鲢作对照）进行了ＲＡＰＤ—ＰＣＲ反应，在２３个引物中除一个引物无扩增产物外，其它均可得到１～１０条ＤＮＡ带，平均每个引物产生５３８条带。其中７个引物产生多态现象，占总引物的３１４％。多态座位达１３６８％。用Ｓｈａｎｎｏｎ指数对ＲＡＰＤ数据进行遗传多样性（Ｈｏ）分析，得出所研究样本的Ｈｏ为０１７５。用血清酯酶和血清蛋白电泳作对比时没有发现在蛋白质水平的多态现象，说明ＲＡＰＤ技术是一种比较灵敏实用的ＤＮＡ多态检测方法。以ＲＡＰＤ所得数据进行了雌核发育鲢子代和普通鲢个体间的遗传相似性与遗传距离分析，为鲢选育提供了依据。
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| 关 键 词： | RAPD，蛋白质电泳，鲢，雌核发育 |
| 修稿时间： | 1998年3月30日 |
GENETIC ANALYSIS OF GYNOGENETIC SILVER CARP( HYPOPHTHALMICHTHYS MOLITRIX) BY MEANS OF RAPD AND PROTEIN ELECTROPHORESIS |
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| Deng Huai,Zhang Siming,Wang Dengqiang and Pan Guangbi.GENETIC ANALYSIS OF GYNOGENETIC SILVER CARP( HYPOPHTHALMICHTHYS MOLITRIX) BY MEANS OF RAPD AND PROTEIN ELECTROPHORESIS[J].Freshwater Fisheries,1998,28(6):10-13. |
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| Authors: | Deng Huai Zhang Siming Wang Dengqiang and Pan Guangbi |
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| Abstract: | Genetic analysis of gynogenetic silver carp including normal silver carp was conducted by means of Random Amplified Polymorphic DNA(RAPD)and protein electrophoresis.23 10 mer primers were used for RAPD PCR reaction.Only one primer did not produce any band.The other 22 primers produced bands ranging from 1 to 10,and on average ,each primer gave rise 1 to 10 bands.Among primers used 7 primers produced polymorphic bands among individual studied.Total 117 bands were produced by 22 primers,and out of those bands(or loci)16(13 68%)bands are polymorphism.Genetic diversity(Ho)was 0 1475 among sample studied.However,no polymorphism was detected by serum protein and Esterase electrophoresis.According to data obtained by RAPD,the genetic similarity and genetic distance among individuals studied are provided.The application of RAPD for carp population genetics and selective breeding was discussed. |
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| Keywords: | RAPD |
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