| 玉米抗大斑病Ht基因有效SSR标记的筛选 |
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| 引用本文： | 王贺,姜敏,刘欣芳,马骏,王和君,王春语,刘祥久.玉米抗大斑病Ht基因有效SSR标记的筛选[J].玉米科学,2011,19(1):48-51. |
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| 作者姓名： | 王贺 姜敏 刘欣芳 马骏 王和君 王春语 刘祥久 |
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| 作者单位： | 辽宁省农业科学院,沈阳,110161 |
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| 基金项目： | 国家转基因生物新品种培育重大专项(2008ZX08003)、辽宁省自然科学基金(20102110) |
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| 摘 要： | 以含有不同抗大斑病基因的鉴别寄主近等基因系为试材,选用分别位于Ht1、Ht2、Ht3、HtN附近的SSR引物进行PCR扩增检测,筛选到Ht1基因有效标记4对,Ht2基因有效标记7对,Ht3基因和HtN基因位点均没有检测到有效标记。
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| 关 键 词： | 玉米 近等基因系 Ht基因 SSR标记 |
| 收稿时间： | 2010/10/12 0:00:00 |
Screening of Effective SSR Molecular Marker for Ht Resistance Gene of Corn Leaf Blight |
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| WANG He,JING Min,LIU Xin-fang,LIU Xiang-jiu,et al..Screening of Effective SSR Molecular Marker for Ht Resistance Gene of Corn Leaf Blight[J].Journal of Maize Sciences,2011,19(1):48-51. |
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| Authors: | WANG He JING Min LIU Xin-fang LIU Xiang-jiu |
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| Institution: | WANG He,JING Min,LIU Xin-fang,LIU Xiang-jiu,et al.(Liaoning Academy of Agricultural Sciences,Shenyang 110161,China) |
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| Abstract: | In this experiment, a set of differential host of near-isogenic lines(NILs) with different resistance gene was used as materials. SSR primers which were located in Ht1, Ht2, Ht3 and HtN nearby were detected for their specific to Ht gene by PCR amplification. We have got four pairs of effective Ht1 gene labeled primers, seven pairs of effective Ht2 gene labeled primers. But no polymorphism was found in Ht3 gene and HtN gene. |
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| Keywords: | Maize NILs Ht gene SSR molecular marker |
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