| 玉米C4型PEPC蛋白的生物信息学分析 |
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| 引用本文： | 崔震海,张立军,李振华,樊金娟,阮燕晔,汪澈.玉米C4型PEPC蛋白的生物信息学分析[J].玉米科学,2009,17(4):135-138. |
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| 作者姓名： | 崔震海 张立军 李振华 樊金娟 阮燕晔 汪澈 |
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| 作者单位： | 沈阳农业大学生物科学技术学院，沈阳 110161 |
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| 基金项目： | 辽宁省科技厅科技攻关项目(2008208001)、沈阳农业大学青年教师科研基金项目(2007)、辽宁省教育厅高校科研计划项目(2008S211)、沈阳市科技局项目(1081191-3-00) |
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| 摘 要： | 利用生物信息学分析软件对GeneBank上注册的玉米C4型PEPC蛋白质序列进行氨基酸组成成分、功能域、二级结构、疏水性、亚细胞定位、导肽及进化树分析和预测。结果表明：玉米PEPC蛋白是等电点5.77的亲水性不稳定蛋白，包含一个CYTH保守结构域，定位于细胞质中，具有中央中间代谢功能，属于连接酶类。α螺旋和不规则卷曲是其蛋白质二级结构的主要结构元件，β折叠和伸展链散布其中；玉米PEPC与高粱、水稻等植物的PEPC亲缘关系较远,不属于同一分支。
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| 关 键 词： | 玉米 PEPC蛋白 结构 功能 进化树 |
| 收稿时间： | 2008/11/3 0:00:00 |
Bioinformatics Analysis on C4 PEPC from Maize |
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| CUI Zhen-hai,ZHANG Li-jun,LI Zhen-hu,FAN Jin-juan,RUAN Yan-ye,WANG Che.Bioinformatics Analysis on C4 PEPC from Maize[J].Journal of Maize Sciences,2009,17(4):135-138. |
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| Authors: | CUI Zhen-hai ZHANG Li-jun LI Zhen-hu FAN Jin-juan RUAN Yan-ye WANG Che |
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| Abstract: | The sequence of C4 PEPC from maize, registered in Gene Bank, was analyzed by bioinformatics tools to predict the following aspects: composition of amino acids, domains, secondary structure, hydrophobicity, subcellular location, leader peptide, functions of protein and phylogenetic analysis. The results showed PEPC was unstable, PI= 5.77, with strong hydrophilicity, including one CYTH domain. PEPC had the function of central intermediary metabolism, classified into ligase; its main secondary structures were alpha helix and random coil and beta sheets and extended strands were sporadic among alpha helix and random coil; C4 PEPC from maize was different from sorghum and rice and they belonged to different branches according to phylogenetic analysis. |
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