| 高效液相色谱法测定膨化玉米制品中的伏马菌素FB_1和FB_2 |
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| 引用本文： | 马丽艳,袁长梅,石文婷,戴蕴青,黄昆仑.高效液相色谱法测定膨化玉米制品中的伏马菌素FB_1和FB_2[J].上海农业学报,2010,26(1):88-91. |
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| 作者姓名： | 马丽艳 袁长梅 石文婷 戴蕴青 黄昆仑 |
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| 作者单位： | 1. 中国农业大学食品科学与营养工程学院,北京,100083;农业部农产品质量监督检验测试中心,北京,100083
2. 农业部农产品质量监督检验测试中心,北京,100083 |
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| 基金项目： | 教育部高等学校特色专业建设项目 |
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| 摘 要： | 采用高效液相色谱荧光检测器（激发波长335nm，发射波长440nm）测定膨化玉米制品中伏马菌素硒和FB2的含量，该方法以乙腈-水溶液（1＋1）为提取溶剂，邻苯二甲醛（OPA）为衍生剂，流动相为甲醇．0．1mol／L磷酸二氢钠（77＋23），流速1．0mL／min。结果表明：衍生反应适宜的pH为9～10，衍生物在0．5～5min内稳定，FB1和FB2的回收率分别为82．5％～93．2％和81．5％～90．7％，最小检出限均为0．03mg／kg。
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| 关 键 词： | 玉米 伏马菌素 液相色谱法 |
Determination of fumonisins in puffed corn products by HPLC |
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| MA Li-yan,YUAN Chang-mei,SHI Wen-ting,DAI Yun-qing,HUANG Kun-lun.Determination of fumonisins in puffed corn products by HPLC[J].Acta Agriculturae Shanghai,2010,26(1):88-91. |
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| Authors: | MA Li-yan YUAN Chang-mei SHI Wen-ting DAI Yun-qing HUANG Kun-lun |
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| Institution: | MA Li-yan1,2,YUAN Chang-mei1,SHI Wen-ting2,DAI Yun-qing1,HUANG Kun-lun1,2(1College of Food Science , Nutrition Engineering,China Agricultural University,Beijing 100083,China,2Supervision & Testing Center for Agricultural Products Quality,Ministry of Agriculture,China) |
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| Abstract: | A fluorescence detector(excitation wavelength 335 nm,emission wavelength 440 nm) was used to determine the fumonisin B_1(FB_1) and fumonisin B_2(FB_2) in puffed corn products by high performance liquid chromatography(HPLC),during which acetonitrile and water solution(1+1) was used as an extractant,OPA as a deriving agent,methanol and 0.1 mol/L sodium dihydrogen phosphate(77+23) as a mobile phase,and the flow rate was 1.0 mL/min.The results showed that the pH value suitable to deriving reaction was 9～10,the derivatives were stable within 0.5～5 min,the recovery rate was 82.5%～93.2% for FB1 and 81.5%～90.7% for FB2,and both detection limits were 0.03 mg/kg. |
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| Keywords: | Corn Fumonisins HPLC |
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