| 鸡白细胞介素-2在毕赤酵母中的表达 |
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| 引用本文： | 杜明华,;吴国平,;张凌晶,;孙乐常,;苏文金,;曹敏杰.鸡白细胞介素-2在毕赤酵母中的表达[J].厦门水产学院学报,2009(1):34-39. |
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| 作者姓名： | 杜明华 ;吴国平 ;张凌晶 ;孙乐常 ;苏文金 ;曹敏杰 |
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| 作者单位： | [1]集美大学生物工程学院,福建厦门361021; [2]福建省高校水产科学技术与食品安全重点实验室,福建厦门361021 |
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| 基金项目： | 福建省自然科学基金资助项目（2006J0418）;厦门市科技计划重点项目（3502220055030） |
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| 摘 要： | 将鸡白细胞介素-2（ChIL-2）基因插入酵母分泌型表达载体pPIC9K构建重组表达载体pPIC9K／ChIL-2，通过电转化构建毕赤酵母（Pichia methanolica）GS115甲醇利用型重组表达菌株．经SDS—PAGE与Western blot分析，证实ChIL-2基因在重组GS115中成功表达出约16ku与14ku两条特异性条带，这与天然ChIL-2相对分子质量大小一致，提示ChIL-2可能为糖基化蛋白质．
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| 关 键 词： | 鸡 白细胞介素-2 毕赤酵母 重组表达 |
Expression of Chicken Interleukin-2 in Pichia methanolica |
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| Institution: | DU Ming-hua, WU Guo-ping, ZHANG Ling-jing, SUN Le-chang, SU Wen-jin, CAO Min-jie(1. School of Biotechnology Engineering, Jimei University, Xiamen 361021, China; 2. Key Laboratory of Science and Technology for Aquaculture and Food Safety (Jimei University) , Fujian Province, Xiamen 361021, China) |
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| Abstract: | ChIL-2 gene was inserted into the expression vector pPIC9K and the recombinant plasmid （pPIC9K/ChIL-2） was transformed into Pichia methanolica GS115 by electroporafion. The expression of recombinant ChIL-2 was successfully performed. Similar to native ChIL-2, GS115 expressed ChIL-2 revealed two main bands corresponding to molecular masses of 16 ku and 14 ku, respectively as detected by SDSPAGE and Western blot, suggesting ChIL-2 was a glycosylated protein. |
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| Keywords: | chicken interleukin-2 （IL-2） Pichia methanolica expression |
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