| DDK品系小鼠遗传特异性的RAPD分析 |
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| 引用本文： | 宋根娣,柴保国,李春丽,赵卫东,周鑫,郑振宇.DDK品系小鼠遗传特异性的RAPD分析[J].河南农业大学学报,2009,43(3). |
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| 作者姓名： | 宋根娣 柴保国 李春丽 赵卫东 周鑫 郑振宇 |
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| 作者单位： | 河南农业大学牧医工程学院,河南郑州,450002 |
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| 基金项目： | 教育部科学技术研究重点项目，河南省教育厅高校杰出科研人才创新工程项目，河南省科技厅重点攻关项目 |
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| 摘 要： | 应用随机扩增多态DNA技术(RAPD)对DDK,C57BL/6,BALB/c,KM,PWK 5个品系的小鼠进行了遗传分析.选用130个随机引物对其基因组DNA进行PCR扩增.根据电泳结果,其中31个引物扩增出明显的多态性条带,共检测到345条扩增片段,其中多态性片段277条,占80.3%,反映了5个品系间的遗传变异.多态性统计分析表明,C57BL/6与BALB/c之间的遗传距离指教为0.013 29,遗传关系最近;DDK与KM,C57BL/6遗传距离指数相似分别为0.014 73,0.0147 6,遗传关系较近,与PWK遗传距离指数为0.016 86,遗传关系最远.结果显示DDK品系小鼠与其他实验室品系之间有遗传的相似性,也存在差异.同时也证明了RAPD技术可以作为分子标记,很好地检测实验室近交系小鼠间的亲缘关系及其遗传特异性.
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| 关 键 词： | DDK品系小鼠 遗传分析 |
RAPD analysis of the genetic specificity of DDK mice |
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| SONG Gen-di,CHAI Bao-guo,LI Chun-li,ZHAO Wei-dong,ZHOU Xin,ZHENG Zhen-yu.RAPD analysis of the genetic specificity of DDK mice[J].Journal of Henan Agricultural University,2009,43(3). |
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| Authors: | SONG Gen-di CHAI Bao-guo LI Chun-li ZHAO Wei-dong ZHOU Xin ZHENG Zhen-yu |
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| Institution: | Engineering College of Animal Husbandry and Veterinary Science;Henan Agricultural University;Zhengzhou 450002;China |
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| Abstract: | Genetic relationship among DDK mice,C57BL/6,BALB/c,KM and PWK were analyzed with randomly amplified polymorphic DNA(RAPD) technique.130 random primers were used to amplify genomic DNA of mice with PCR methods.On the basis of preliminary experiments,31 primers were selected to analyze the genetic variation among 5 mice lines.345 amplified fragments were detected by electrophoresis on agarose gel.Among them,277 fragments were polymorphic,accounting for 80.3% of the whole.It was shown that the RAPD polymorphis... |
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| Keywords: | RAPD |
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