| 鸡传染性支气管炎病毒HN99株膜蛋白基因的分子特征分析 |
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| 引用本文： | 贺秀媛,张淑霞,张志平,张君涛,陈红英,王川庆,崔保安,王建华.鸡传染性支气管炎病毒HN99株膜蛋白基因的分子特征分析[J].河南农业大学学报,2010,44(2). |
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| 作者姓名： | 贺秀媛 张淑霞 张志平 张君涛 陈红英 王川庆 崔保安 王建华 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,河南,郑州,450002
2. 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
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| 基金项目： | 河南省科技攻关项目，陕西省自然科学基金 |
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| 摘 要： | 根据GenBank中已经公布的鸡传染性支气管炎病毒株M基因序列,设计并合成1对特异引物,利用RT-PCR方法扩增出IBV河南分离株HN99株M基因的全长片段,然后进行克隆、测序,获得了长度为669 bp的HN99株M基因全序列,编码M蛋白222个氨基酸残基,其N端的前60 nt为前导序列,近N端含有1个潜在的N-糖基化位点,M基因编码的膜蛋白中含有9个高度保守的半胱氨酸;HN99株M蛋白点突变较多,且突变位点呈散在分布遍及整个ORF,主要表现为碱基的插入和缺失,在Glu 21～Leu 37,Set 45～Ile 68,Pro 74～Phe 94位氨基酸的区域内形成3个跨膜区域,在39 Try～43 Thr,171 Ile～176 Pro,183 Arg～193 Ser住氨基酸处含有3个潜在的抗原位点;与GenBank中的11个国内外参考毒株相比,IBV-HN99株M基因核苷酸同源性为87.0%～90.3%,推导的氨基酸同源性为89.7%～94.2%;在系统发生进化树中,HN99株M基因与其他参考毒株属于不同的分支,亲缘关系均较远.
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| 关 键 词： | 鸡 传染性支气管炎病毒 HN99株 M基因 分子特征 |
Molecular characteristics of matrix glycoprotein gene of infectious bronchitis virus HN99 isolated |
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| HE Xiu-yuan,ZHANG Shu-xia,ZHANG Zhi-ping,ZHANG Jun-tao,CHEN Hong-ying,WANG Chuan-qing,CUI Bao-an,WANG Jian-hua.Molecular characteristics of matrix glycoprotein gene of infectious bronchitis virus HN99 isolated[J].Journal of Henan Agricultural University,2010,44(2). |
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| Authors: | HE Xiu-yuan ZHANG Shu-xia ZHANG Zhi-ping ZHANG Jun-tao CHEN Hong-ying WANG Chuan-qing CUI Bao-an WANG Jian-hua |
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| Institution: | 1.Engineering College of Animal Husbandry and Veterinary Science/a>;Henan Agricultural University/a>;Zhengzhou 450002/a>;China/a>;2.College of Animal Science and Veterinary Science/a>;Northwest Agricultural and Forestry University/a>;Yangling 712100/a>;China |
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| Abstract: | The complete matrix glycoprotein gene of an infectious bronchitis virus strain HN99 isolated from Henan was amplified by RT-PCR method using a pair of specific primers designed according to the published Matrix glycoprotein gene sequence of IBV,The PCR products were cloned into the pMD18-T vector.Positive clones were identified by the restriction endonuclease analysis and PCR. The gene was sequenced and compared with 11 reference IBV strains from the GenBank. The results showed that the full length sequence... |
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| Keywords: | chicken infectious bronchitis virus HN99 strain matrix glycoprotein gene molecular characteristics |
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