| 佛手组织培养和Km敏感性试验 |
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| 引用本文： | 周春丽,郭卫东,张燕燕.佛手组织培养和Km敏感性试验[J].西北林学院学报,2005,20(4):76-79. |
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| 作者姓名： | 周春丽 郭卫东 张燕燕 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西,杨陵,712100;浙江师范大学,化学与生命科学学院,浙江,金华,321004
2. 浙江师范大学,化学与生命科学学院,浙江,金华,321004 |
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| 基金项目： | 浙江省科技计划项目（2003C32043）. |
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| 摘 要： | 以佛手的叶片、茎段为材料,建立了其组织培养系统。研究表明,B5比M S更适合作为诱导佛手愈伤组织的基础培养基,诱导愈伤组织效率较高的培养基配方为B5+2.0 m g.L-12,4-D+0.2 m g.L-1ZT和B5+1.5 m g.-L 12,4-D+1.0 m g.L-1NAA+0.4 m g.L-16-BA。在叶片对卡那霉素敏感性的试验中发现,叶片外植体预培养2 d后进行筛选的效果较好,卡那霉素的适宜筛选浓度为25 m g.L-1。
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| 关 键 词： | 佛手 组织培养 卡那霉素 |
| 文章编号： | 1001-7461（2005）04-0076-04 |
| 收稿时间： | 2005-05-20 |
| 修稿时间： | 2005年5月20日 |
Selective Test of Sensitivity to Kanamycin and Tissue Culure of Citrus medica, var. sarcodactylis |
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| ZHOU Chun-li,GUO Wei-dong,ZHANG Yan-yan.Selective Test of Sensitivity to Kanamycin and Tissue Culure of Citrus medica, var. sarcodactylis[J].Journal of Northwest Forestry University,2005,20(4):76-79. |
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| Authors: | ZHOU Chun-li GUO Wei-dong ZHANG Yan-yan |
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| Institution: | ZHOU Chun-li~1,GUO Wei-dong~2,ZHANG Yan-yan~2 |
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| Abstract: | The preliminary results of tissue culture of bergamot were reported in this paper.The results showed that B_5 medium was more suitable for callus inducement than MS medium.Leaves and stem segments with axillary buds of bergamot were used as explants,and a tissue culture system were established.B_5+2.0 mg/L 2,4-D +0.2 mg/L ZT and B_5+1.5 mg/L 2,4-D+1.0 mg/L NAA+0.4 mg/L 6-BA media were more suitable for callus inducement.The appropriate concentration of kanamycin for selection of transformed leaf was 25 mg/L. |
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| Keywords: | Citrus rnedica var sarcodactylis tissue culture kanamycin |
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