| 忍冬茎叶中绿原酸的提取工艺研究 |
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| 引用本文： | 张珺,张树军,李娜,王俊儒.忍冬茎叶中绿原酸的提取工艺研究[J].西北林学院学报,2012,27(3):137-141,268. |
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| 作者姓名： | 张珺 张树军 李娜 王俊儒 |
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| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西杨陵,712100
2. 西北农林科技大学理学院,陕西杨陵,712100 |
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| 基金项目： | 2005年度教育部新世纪优秀人才支持计划(NCET-05-0855) |
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| 摘 要： | 以高效液相色谱为检测手段,绿原酸提取率为考察指标,比较了回流法和超声法提取忍冬茎叶中绿原酸的影响;采用单因素和正交试验设计,探讨了回流法提取忍冬茎叶中绿原酸的最佳工艺参数。结果表明,最佳提取条件为乙醇浓度70%,料液比1∶14,提取时间2h,提取温度60℃,提取溶剂最初pH 6,此时绿原酸提取率最高,可达1.37%。
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| 关 键 词： | 忍冬茎叶 绿原酸 正交试验 高效液相色谱 |
Extraction Process of Chlorogenic Acid from the Stems and Leaves of Lonicera japonica |
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| ZHANG Jun
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ZHANG Shu-jun
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LI Na
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WANG Jun-ru.Extraction Process of Chlorogenic Acid from the Stems and Leaves of Lonicera japonica[J].Journal of Northwest Forestry University,2012,27(3):137-141,268. |
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| Authors: | ZHANG Jun ZHANG Shu-jun LI Na WANG Jun-ru |
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| Institution: | 1.College of Life Sciences,Northwest A&F University,Yangling,Shaanxi 712100,China; 2.College of Science,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | Two methods were compared to extract chlorogenic acid from the stems and leaves of Lonicera japonica,alcohol reflux extraction and ultrasound-assisted maceration extraction.The content of chlorogenic acid was used as index,which was measured by high performance liquid chromatography.Single factor and orthogonal design experiments were adopted to select optimal extraction conditions in alcohol reflux extraction.The results showed that under the same concentrations of ethanol,reflux extraction was better than ultrasound-assisted maceration extraction.Optimal reflux extraction conditions were as follows: solvent: 70% ethanol,ratio of material to solvent(W∶V): 1∶14,reflux time: 2 h,extraction temperature: 60℃,and pH 6.Under these conditions,the extraction ratio of chlorogenic acid was up to 1.37%. |
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| Keywords: | stems and leaves of Lonicera japonica chlorogenic acid orthogonal test HPLC |
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