| 电转化法及其在苏云金杆菌中的应用 |
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| 引用本文： | 陈建武,黄必旺,黄志鹏,林毅,关雄.电转化法及其在苏云金杆菌中的应用[J].福建农林大学学报(自然科学版),1999,28(3):309-314. |
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| 作者姓名： | 陈建武 黄必旺 黄志鹏 林毅 关雄 |
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| 作者单位： | 福建农业大学生物技术中心,福州,350002 |
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| 基金项目： | 国家重点科技攻关项目，“九五”国家重点科技攻关项目，福建省计委资助 |
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| 摘 要： | 苏云金杆菌（ Ｂｔ）工程菌的构建，可以采用原生质体融合、接合转移和转化等方法，其中电转化法被公认为是最有效的手段之一，此技术已广泛地应用于 Ｂｔ的遗传改良．本文阐述了电转化基本原理及基本操作步骤．此外，还就存在的问题和应用前景进行探讨
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| 关 键 词： | 苏云金芽孢杆菌 电转化 遗传改良 |
| 修稿时间： | 1999－03－18 |
Electroporation and its application to Bacillus thuringiensis |
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| Chen Jianwu,Huang Biwang,Huang Zhipeng,Lin Yi,Guan Xiong.Electroporation and its application to Bacillus thuringiensis[J].Journal of Fujian Agricultural and Forestry University,1999,28(3):309-314. |
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| Authors: | Chen Jianwu Huang Biwang Huang Zhipeng Lin Yi Guan Xiong |
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| Abstract: | Engineering strains of Bacillus thuringiensis (Bt) can be constructed by protoplast fusion, conjugation transfer and electroporation, of which electroporation has been considered as the most effective method and extensively applied to genetic transformation of Bt. In this paper its principles and major manipulations are dealt with, and the problems and prospects of application are also discussed. |
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| Keywords: | Bacillus thuringiensis electroporation genetic transformation |
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