| 福建乌龙茶种质离体保存研究Ⅰ.成年茎段无菌系建立 |
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| 引用本文： | 郭玉琼,赖钟雄,吕柳新,林腾辉,陈菁.福建乌龙茶种质离体保存研究Ⅰ.成年茎段无菌系建立[J].福建农林大学学报(自然科学版),2008,37(6). |
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| 作者姓名： | 郭玉琼 赖钟雄 吕柳新 林腾辉 陈菁 |
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| 作者单位： | 1. 福建农林大学园艺植物生物工程研究所,福建,福州福建农林大学园艺学院,福建,福州,350002
2. 福建农林大学园艺学院,福建,福州,350002 |
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| 基金项目： | 福建省果茶重大科技专项，福建省科技厅重点项目 |
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| 摘 要： | 以代表性乌龙茶品种铁观音成年新梢茎段为外植体,初步探讨了茶树成年茎段离体培养无菌系的建立.结果表明,外植体的适宜消毒方法为0.1%升汞浸泡11 min;适宜取材气候条件是连续晴天5 d以上;适宜取材部位是第二腋芽的茎段;适宜的诱导培养基为MS+2.0 mg.L-16-BA+0.5 mg.L-1IAA+0.5 mg.L-1GA3.
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| 关 键 词： | 乌龙茶 铁观音 成年茎段 无菌系 离体保存 |
In vitro preservation of germplasm of oolong tea trees (Camellia sinensis) in FujianⅠ. Establishment of aseptic lines from adult stem nodes |
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| GUO Yu-qiong,LAI Zhong-xiong,LV Liu-xin,LIN Teng-hui,CHEN Jing.In vitro preservation of germplasm of oolong tea trees (Camellia sinensis) in FujianⅠ. Establishment of aseptic lines from adult stem nodes[J].Journal of Fujian Agricultural and Forestry University,2008,37(6). |
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| Authors: | GUO Yu-qiong LAI Zhong-xiong LV Liu-xin LIN Teng-hui CHEN Jing |
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| Abstract: | In this experiment the stem nodes from young shoots of adult trees were used as materials for the preliminary study on the optimum conditions for the establishment of aseptic lines from adult stem nodes and proliferation in Camellia sinensis cv Tieguanyin.The results showed that the optimal sterilizing time of 0.1% HgCl2 was 11 min for explants;the best weather condition of sampling explants was continuously sunny for 5 d;the best explants were the stem nodes with second auxiliary bud;the best medium for bud germination was MS medium supplemented with 2.0 mg·L-1 6-BA,0.5 mg·L-1 IAA and 0.5 mg·L-1 GA3. |
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| Keywords: | oolong tea tree Tieguanyin stem nodes from adult trees aseptic line in vitro preservation |
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