| 基因枪转化甘蔗愈伤组织体系的优化 |
| |
| 引用本文： | 陈平华,陈如凯.基因枪转化甘蔗愈伤组织体系的优化[J].福建农林大学学报(自然科学版),2004,33(3):355-358. |
| |
| 作者姓名： | 陈平华 陈如凯 |
| |
| 作者单位： | 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 |
| |
| 基金项目： | 国家高技术研究发展计划资助项目(2001AA241191，2002AA241031)，福建省青年科技人才创新资助项目(2001J044). |
| |
| 摘 要： | 以生产上广泛应用的甘蔗商业品种新台糖16号的愈伤组织为受体材料,以含有GUS基因的植物表达载体pBI121为外源DNA,利用基因枪PDS 1000/He,设定不同的参数,将pBI121轰击新台糖16号愈伤组织.经GUS染色,结果发现在发射距离1/4in、可裂膜压力1100psi、轰击距离6cm时转化效率最高.
|
| 关 键 词： | 甘蔗 愈伤组织 植物表达载体 PDS1000/He GUS染色 |
| 文章编号： | 1006-7817(2004)03-0355-04 |
| 修稿时间： | 2004年3月10日 |
Optimizing of genetic transformation of sugarcane calli via PDS-1000/He |
| |
| CHEN Ping-hua,CHEN Ru-kai iculture,P.R.China,Fujian Agriculture and Forestry University,Fuzhou,Fujian ,China.Optimizing of genetic transformation of sugarcane calli via PDS-1000/He[J].Journal of Fujian Agricultural and Forestry University,2004,33(3):355-358. |
| |
| Authors: | CHEN Ping-hua CHEN Ru-kai iculture PRChina Fujian Agriculture and Forestry University Fuzhou Fujian China |
| |
| Institution: | CHEN Ping-hua,CHEN Ru-kai iculture,P.R.China,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
| |
| Abstract: | Calli of sugarcane cultivar "ROC"16 were selected as the targets for bombardment. The plant expression vector pBI121 with GUS gene was selected as the extrinsic DNA to coat microcarriers. The bombardment parameters of PDS-1000/He were adjusted and the calli were bombed. The calli were assayed with a colorimetric GUS assay. The results showed that the efficiency of transformation was high when distance between rupture disk and macrocarrier, the rupture pressure, distance between stopping screen and target shelf were 1/4 inch, 1 100 psi, 6 cm, respectively. |
| |
| Keywords: | sugarcane calli plant expression vector PDS-1000/He colorimetric GUS assay |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
