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| 应用RAPD技术快速鉴定樱桃番茄杂种纯度 | |
| 引用本文： | 温庆放,朱海生,林义章,康建坂,薛珠政.应用RAPD技术快速鉴定樱桃番茄杂种纯度[J].福建农林大学学报(自然科学版),2006,35(1):33-37. |
| 作者姓名： | 温庆放 朱海生 林义章 康建坂 薛珠政 |
| 作者单位： | 1. 福建省农业科学院蔬菜研究中心,福建,福州,350013 2. 福建省农业科学院蔬菜研究中心,福建,福州,350013;福建农林大学园艺学院,福建,福州,350002 3. 福建农林大学园艺学院,福建,福州,350002 |
| 摘 要： | 筛选出应用于樱桃番茄种子纯度鉴定的引物,建立了杂交种子的RAPD指纹图谱.从27条引物中筛选出了18条用于樱桃番茄种子纯度鉴定的引物,其中4条偏母型引物、5条偏父型引物、4条互补型引物、2条特异型引物及3条缺失型引物.利用互补型引物S172鉴定21粒樱桃番茄种子纯度,其中有2个其他种子混杂,该种子纯度为90.48%. |
| 关 键 词： | 樱桃番茄 RAPD 杂种 鉴定 |
| 文章编号： | 1671-5470(2006)01-0033-05 |
| 收稿时间： | 2005-03-11 |
| 修稿时间： | 2005-11-29 |
Hybrid purity identification of Lycopersicon esculentum var. cerasiforme germplasms by RAPD analysis |
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| WEN Qing-fang,ZHU Hai-sheng,LIN Yi-zhang,KANG Jian-ban,XUE Zhu-zheng.Hybrid purity identification of Lycopersicon esculentum var. cerasiforme germplasms by RAPD analysis[J].Journal of Fujian Agricultural and Forestry University,2006,35(1):33-37. | |
| Authors: | WEN Qing-fang ZHU Hai-sheng LIN Yi-zhang KANG Jian-ban XUE Zhu-zheng |
| Institution: | 1. Research Center of the Vegetable Science, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China; 2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China |
| Abstract: | The primers were screened for the purity identification of Lycopersicon esculentum var.cerasiforme hybrid seeds,and the fingerprints of RAPD were established among the parents and their hybrid seeds.From 27 primers,18 primers were screened and proved suitable for the purity identification.Among them,4 primers displayed partial female type,5 primers partial male type,4 primers complementary type,2 primers special,3 type primers shortage type.Using complementary type primer S172,2 fasle hybrid seeds were identified from 21 ones.Therefore,the purity of L.esculentum var.cerasiforme hybrid seeds was 90.48%. |
| Keywords: | RAPD |
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