| 印楝素干粉的制备及生物活性测定 |
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| 引用本文： | 李一鸣,陈孝甜,王勇庆,程东美,张志祥.印楝素干粉的制备及生物活性测定[J].华南农业大学学报,2017,38(4):48-51. |
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| 作者姓名： | 李一鸣 陈孝甜 王勇庆 程东美 张志祥 |
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| 作者单位： | 1. 天然农药与化学生物学教育部重点实验室/华南农业大学 农学院,广东 广州,510642;2. 忡恺农业工程学院 农学院,广东 广州,510225 |
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| 基金项目： | 广东省自然科学基金（2016A030313387） |
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| 摘 要： | 【目的】测定印楝素干粉及印楝素A、印楝素B的生物活性,建立印楝素干粉中印楝素含量的检测方法。【方法】采用乙酸乙酯浸提法制备印楝素干粉,HPLC法检测印楝素含量,叶碟法测定生物活性。【结果】处理24 h后,印楝素干粉对斜纹夜蛾Spodoptera litura 2龄幼虫和大菜粉蝶Pieris brassicae 3龄幼虫的AFC50值分别为0.28和70.71μg·m L~(-1),拒食活性明显高于印楝素A和印楝素B。【结论】该干粉制备方法不仅简单有效,且生物活性良好,具有推广应用价值。
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| 关 键 词： | 印楝素干粉 斜纹夜蛾 大菜粉蝶 乙酸乙酯浸提法 叶碟法 HPLC 生物活性 |
| 收稿时间： | 2017/3/14 0:00:00 |
Preparation and bioactivity determination of azadirachtin-rich dry powder |
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| LI Yiming,CHEN Xiaotian,WANG Yongqing,CHENG Dongmei and ZHANG Zhixiang.Preparation and bioactivity determination of azadirachtin-rich dry powder[J].Journal of South China Agricultural University,2017,38(4):48-51. |
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| Authors: | LI Yiming CHEN Xiaotian WANG Yongqing CHENG Dongmei and ZHANG Zhixiang |
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| Institution: | 1 Key Laboratory of Natural Pesticide and Chemical Biology/College of Agriculture, South China Agricultural University, Ministry of Education,,1 Key Laboratory of Natural Pesticide and Chemical Biology/College of Agriculture, South China Agricultural University, Ministry of Education,,1 Key Laboratory of Natural Pesticide and Chemical Biology/College of Agriculture, South China Agricultural University, Ministry of Education,,2 College of Agriculture, Zhongkai University of Agriculture and Engineering, and 1 Key Laboratory of Natural Pesticide and Chemical Biology/College of Agriculture, South China Agricultural University, Ministry of Education, |
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| Abstract: | Objective] To study the biological activity of azadirachtin-rich dry powder, azadirachtin A and azadirachtin B, and establish a method for determinating azadirachtin content of dry powder.Method] Azadirachtin-rich dry powder was prepared by ethyl acetate extraction method, the azadirachtin content of dry powder was determined by HPLC and the biological activity was measured by leaf disc test.Result]AFC50 of zadirachtin-rich dry powder was 0.28 μg·mL-1 against 2nd instar larvae of Spodoptera litura and 70.71 μg·mL-1 against the 3rd instar larvae of Pieris brassicae 24 h after treatment.The antifeedant activity of azadirachtin-rich dry powder was clearly higher than that of azadirachtin A and azadirachtin B.Conclusion] This preparation method of the dry powder is simple, effective, and has good biological activity.It is recommended for popularization and application. |
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| Keywords: | azadirachtin-rich dry powder Spodoptera litura Pieris brassicae ethyl acetate extraction method leaf disc test HPLC bioacitivity |
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