| 小番茄内生菌耐药基因检测及种植模型中GFP标记菌转移研究 |
| |
| 引用本文： | 钟薇馨,石珂弋,陈意群,王晓佩,陶皖豫,马凯雄,孙永学.小番茄内生菌耐药基因检测及种植模型中GFP标记菌转移研究[J].华南农业大学学报,2018,39(4):55-60. |
| |
| 作者姓名： | 钟薇馨 石珂弋 陈意群 王晓佩 陶皖豫 马凯雄 孙永学 |
| |
| 作者单位： | 广东省兽药研制与安全评价重点实验室/国家兽药安全评价(环境评估)实验室/华南农业大学兽医学院 |
| |
| 基金项目： | 广东省自然科学基金重点项目（2016A030311029）；广东省大学生科技创新训练项目（201610564121） |
| |
| 摘 要： | 【目的】调查广州市售小番茄内生菌中常用抗菌药物耐药基因流行情况及基于绿色荧光蛋白(GFP)标记的大肠埃希菌人工栽种模型探讨耐药基因转移进入小番茄果实内的可能性。【方法】采用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)法分离鉴定市售小番茄内生菌,PCR定性检测内生菌中氯霉素类(cmlA)、四环素类(tetA和tetM)、磺胺类(sul1、sul2和sul3)和喹诺酮类(oqxA、oqxB、qnrB、qnrS和qepA)等药物耐药基因的流行情况;采用GFP标记–大肠埃希菌人工种植模型检测小番茄果实、叶片和根系中目标GFP菌,探讨内生菌及GFP标记进入小番茄内部的可能性。【结果】市售小番茄分离的52株内生菌菌株中,肠杆菌属Enterobacter和克雷伯菌属Klebsiella占有较高比例,几乎所有菌株均携带oqx B基因(总阳性率达92.31%),在人工种植模型第27天采集的果实中分离出含有GFP标记的内生菌。【结论】小番茄内生菌中oqx B基因最为流行,耐药基因可通过根系浇灌的方式转移到小番茄果实内。
|
| 关 键 词： | 小番茄 种植模型 耐药基因 GFP标记-大肠埃希菌 内生菌 |
| 收稿时间： | 2017/11/24 0:00:00 |
Detection of antibiotic resistant genes in cherry tomato entophytic bacteria and transfer of GFP marked bacteria in plantation model |
| |
| ZHONG Weixin,SHI Keyi,CHEN Yiqun,WANG Xiaopei,TAO Wanyu,MA Kaixiong and SUN Yongxue.Detection of antibiotic resistant genes in cherry tomato entophytic bacteria and transfer of GFP marked bacteria in plantation model[J].Journal of South China Agricultural University,2018,39(4):55-60. |
| |
| Authors: | ZHONG Weixin SHI Keyi CHEN Yiqun WANG Xiaopei TAO Wanyu MA Kaixiong and SUN Yongxue |
| |
| Institution: | Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China,Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China and Guangdong Provincial Key Laboratory for Veterinary Pharmaceutics Development and Safety Evaluation/National Laboratory of Veterinary Drug Safety Evaluation(Environment Estimation)/College of Veterinary, South China Agricultural University, Guangzhou 510642, China |
| |
| Abstract: | |
| |
| Keywords: | cherry tomato plantation model antibiotic resistant gene GFP-Escherichia coli entophytic bacterium |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《华南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《华南农业大学学报》下载免费的PDF全文 |
|
