| 牛血清白蛋白在锁阳RAPD分析中的作用 |
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| 引用本文： | 周玉碧,叶润蓉,卢学峰,刘洋,彭敏.牛血清白蛋白在锁阳RAPD分析中的作用[J].农业科学与技术,2007,8(3):64-67. |
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| 作者姓名： | 周玉碧 叶润蓉 卢学峰 刘洋 彭敏 |
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| 作者单位： | [1]中国科学院西北高原生物研究所,青海西宁810001 [2]中国科学院研究生院,北京100039 [3]青海省青藏高原特色生物资源研究重点实验室,青海西宁810001 |
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| 基金项目： | 中国科学院知识创新工程项目 |
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| 摘 要： | 由于植物DNA常含有内源多酚化合物，影响其PCR扩增。而研究表明，牛血清白蛋白（Bovine serum albumin，BSA）被应用于酶及PCR反应系统中，可以起到减少内源抑制物的干扰作用。为此，笔者研究了BSA在锁阳RAPD分析中的作用。
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| 关 键 词： | BSA Cynomorium sortgaricum RAPD 牛血清白蛋白 锁阳 RAPD Analysis 分析 作用 provided foundation application feasible improvement plants concentration used effect dosage enzyme process RAPD analysis Result amplification |
| 修稿时间： | 2007年10月31 |
Effect of BSA on RAPD Analysis of Cynomorium songaricum |
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| ZHOU Yu-bi,YE Run-rong,LU Xue-feng,LIU Yang,PENG Min.Effect of BSA on RAPD Analysis of Cynomorium songaricum[J].Agricultural Science ＆ Technology,2007,8(3):64-67. |
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| Authors: | ZHOU Yu-bi YE Run-rong LU Xue-feng LIU Yang PENG Min |
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| Abstract: | Objeetive]The objective of the study was to study the role played by BSA in Random Amplified polymorphic DNA(RAPD).Method]The improvement of RAPD amplification was observed by adding BSA in the analysis of Cynomorium songaricum RAPD.Result]The result showed that in the process of Cynomorium songaricum RAPD analysis,the effect of amplification impmved significantly and the dosage of Taq enzyme declined by adding BSA.The suitable concentration of BSA used in RAPD of Cynomorium songaricum Rupr was 2 μg/μl. Conclusion]It is feasible to add BSA to improve the RAPD analysis of plants. This study has provided foundation for the application of BSA in RAPD analysis. |
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| Keywords: | BSA Cynomorium sortgaricum RAPD |
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