Genetic Relationship Analysis of Ramie Accessions by RAPD Markers

Genetic relationship of 12 ramie varieties with different drought resistance was analysed by using RAPDI Twenty-five 10- mer random primers were used to amplify the total DNA of these varieties. The results ahowed that the amplified products of 12 ramie varieties could be obtained with 12 primers. The length of amplified products was 0.6- 5.15 kb. Ninety bands were identified in total, of which 11 bands were common in all 12 varieties and 79 bands were polymorphic. The polymorphic bands were 87.78% of the total amplified bands. According to the result of cluster analysis, 12 ramie varieties could be divided into two classes and three groups. It was found that high drought resistant varieties were clustered into different groups or subgroups in the same class, which shows near relationship among them.

Genetic Relationship Analysis of Ramie Accessions by RAPD Markers

Genetic relationship of 12 ramie varieties with different drought resistance was analysed by using RAPD. Twenty-five 10- mer random primers were used to amplify the total DNA of these varietiss. The results showed that the amplified products of 12 ramie varieties could be obtained with 12 primers. The length of amplified products was 0.6 - 5.15 kb. Ninety bands were identified in total, of which 11 bands were common in all 12 varieties and 79 bands were polymrphic. The polymorphic bands were 87.78 9% of the total amplified bands. According to the result of cluster analysis, 12ramie varieties could be divided into two classes and three groups. It was found that high drought resistant varieties were clustered into different groups or subgroups in the same class, which shows near relationship among them.