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西瓜枯萎病土壤拮抗菌的筛选(英文)
引用本文:胡雪芹,张洪斌,苏晓飞.西瓜枯萎病土壤拮抗菌的筛选(英文)[J].农业科学与技术,2008,9(6):132-135.
作者姓名:胡雪芹  张洪斌  苏晓飞
作者单位:合肥工业大学制药工程系,安徽合肥,230009 
基金项目:合肥工业大学科学研究发展基金,安徽省高等学校青年教师科研资助计划 
摘    要:Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil.Method] Actinomycete strains,fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method,then its resistance was screened respectively by the improved confront culture method after colonies were purified.Finally,bacteria strains with better antagonistic effect were identified.Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples,which contained 15 fluorescent bacteria strains,5 bacillus strains and 9 actinomycete strains.Furthermore,three antagonistic bacteria strains of FM2,FM3 and FM4 with the strongest antagonism were identified primarily.Conclusion] According to cultural characteristics,morphological observation,biochemical and physiological tests,FM2 belongs to bacillus subtilis,while FM3 and FM4 belong to micrococcus.

关 键 词:Antagonistic  bacteria  Watermelon  fusarium  wilt  Bacillius  Micrococcus

Screening of Soil Antagonistic Bacteria for Watermelon Fusarium Wilt
HU Xue-qin,ZHANG Hong-bin,SU Xiao-fei.Screening of Soil Antagonistic Bacteria for Watermelon Fusarium Wilt[J].Agricultural Science & Technology,2008,9(6):132-135.
Authors:HU Xue-qin  ZHANG Hong-bin  SU Xiao-fei
Institution:Department of Pharmaceutical Engineering,Hefei University of Technology,Hefei 230009
Abstract:Objective] The aim of this study was to screen bacteria strains with stable antagonistic effect against watermelon fusarium wilt from soil and investigate the biological control of watermelon fusarium wilt by applying the antagonistic bacteria strains into soil. Method] Actinomycete strains, fluorescent bacteria strains and bacillus strains were isolated from soil samples by the dilution-plate method, then its resistance was screened respectively by the improved confront culture method after colonies were purified. Finally, bacteria strains with better antagonistic effect were identified. Result] 29 bacteria strains with stable antagonistic effect against watermelon fusarium wilt were screened from 39 soil samples, which contained 15 fluorescent bacteria strains, 5 bacillus strains and 9 actinomycete strains. Furthermore, three antagonistic bacteria strains of FM2, FM3 and FM4 with the strongest antagonism were identified primarily. Conclusion] According to cultural characteristics, morphological observation, biochemical and physiological tests, FM2 belongs to bacillus subtilis, while FM3 and FM4 belong to micrococcus.
Keywords:Antagonistic bacteria  Watermelon fusarium wilt  Bacillius  Micrococcus
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