| 抗A型禽流感病毒核蛋白特异性单克隆抗体研究 |
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| 引用本文： | 李娜,秦爱建,邵红霞,金文杰,刘岳龙.抗A型禽流感病毒核蛋白特异性单克隆抗体研究[J].农业科学与技术,2008,9(1):60-63. |
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| 作者姓名： | 李娜 秦爱建 邵红霞 金文杰 刘岳龙 |
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| 作者单位： | 扬州大学兽医学院,江苏扬州225009 |
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| 基金项目： | 国家科技攻关计划
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江苏省科技攻关计划 |
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| 摘 要： | 禽流感（AI）是由A型禽流感病毒（AIV）引起的一种发生于禽类的病毒性传染病。笔者以杂交瘤技术研制抗AIV共同抗原的特异单克隆抗体，旨在建立一种双抗体夹心ELISA方法快速检测AIV，以便为A型AIV的快速诊断技术的研究提供理论依据。
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| 关 键 词： | 禽流感 抗A型禽流感病毒核蛋白 特异性 单克隆抗体 |
Development and Characterization of Monoclonal Antibody Specific to Nuclear Protein of Avian Influenza Virus Type A |
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| LI Na,QIN Ai-jian,SHAO Hong-xia,JIN Wen-jie,LIU Yue-long.Development and Characterization of Monoclonal Antibody Specific to Nuclear Protein of Avian Influenza Virus Type A[J].Agricultural Science ＆ Technology,2008,9(1):60-63. |
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| Authors: | LI Na QIN Ai-jian SHAO Hong-xia JIN Wen-jie LIU Yue-long |
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| Institution: | (College of VeterinaryMedicine, Yangzhou University, Yangzhou 225009) |
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| Abstract: | Five monoclonal antibodies(Mabs)to nuclear protein of avain influenza virus(AIV)were developed by syncretizing SP 2/0 and the spleen ceils from BALB of mice immuized with H9 subtype AIV.Specificity of these Mabs were identified by immunofluorescont assay(IFA)and enzyme linked immunesorbent assay(ELISA).These five Mabs which were named as AIV-NP-2C3,AIV-NP-6A5,AIV-NP-3H9,AIV-NP-TB4,AIV-NP-2H4 could react with all viruses of AIV-H9 strains in tests.The result of Western blotting showed that only the 60 ku protein antigen of AIV-H9 could be recognized by the Mabs but never recognized by Now castle disease virus,REV and infectious bursa disease virus.The result of preliminary application showed that avian influenza viruses could be dcetectod by Mabs in IFA and ELISA.All these Mabs will probably play important roles in preventing and monitoring avian influenza viruses. |
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| Keywords: | Avian influenza virus NP Mouocloual antibody Immunofluorescont assay(IFA) ELISA |
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