高效液相色谱法分离和测定油茶茎尖组织中4种内源激素

建立了高效液相色谱法测定油茶茎尖组织中生长素(IAA)、赤霉素(GA3)、玉米核苷(ZR)、脱落酸(ABA)4种内源激素的方法。应用反相C18色谱柱,以甲醇-水(含0.75%冰乙酸,35∶65,V/V)为流动相,流速为0.8 ml/min,进样量10μl,检测波长254 nm,柱温30℃,该方法各峰分离效果理想。IAA、GA3、ZR、ABA的最低检出限分别为0.05、0.01、0.02、0.01 mg/L,回收率分别为98.2%、97.9%、96.3%、93.4%。该方法易操作且准确性高。

Determination of Four Endogenous Hormones in Camellia oleifera Shoot- tip Tissues by HPLC

A reversed-phase high performance liquid chromatography(RP-HPLC) method was developed for the determination of indole-3-acetic acid(IAA),gibberellin acid(GA3),zeatin riboside(ZR),and abscisic acid(ABA) in Camellia oleifera shoot-tip tissues.The method was performed with a reverse-phase C18 column and using the mixture of methanol and water(containing 0.75% acetic acid) with the volume ratio of 35∶65 as mobile phase;the sample injection volume was 10 μl and the flow rate was 0.8 ml/min;the detection wavelength was 254 nm and the column temperature was 30℃.These hormones were separated well by this method.The lowest detection limit of IAA,GA3,ZR and ABA were 0.05,0.01,0.02,and 0.01 mg/L respectively and the recovery rate were 98.2%,97.9%,96.3% and 93.4% respectively.In a word,the method was convenient and accurate.