| 生防真菌球毛壳ND35的RAPD特异序列的标记转换 |
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| 引用本文： | 戴杨,温丽伟,高克祥,何邦令,吴海燕,刘畅.生防真菌球毛壳ND35的RAPD特异序列的标记转换[J].山东农业科学,2011(10):8-11. |
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| 作者姓名： | 戴杨 温丽伟 高克祥 何邦令 吴海燕 刘畅 |
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| 作者单位： | 山东农业大学植物保护学院,山东泰安,271018 |
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| 基金项目： | 国家自然科学基金，公益性行业(农业)科研专项项目，山东省科技发展计划项目 |
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| 摘 要： | 利用随机扩增多态性DNA（RAPD）方法对包括球毛壳ND35在内的20个真菌分离物进行分析，筛选出球毛壳ND35的RAPD扩增特异性DNA片段，经pEASY—T3载体克隆和测序、Seqman整合后，用Primer5．0软件设计了球毛壳ND35的特异引物对CgND35—1：GTTGCCAGCCCGAGGGGAAG，CgND35—2：G111ACCAGCCATGCCTTGAAG，经优化PCR条件，成功获得球毛壳ND35的SCAR标记，该标记片段长度为329bp。用CgND35—1／CgND35—2引物对进行了SCAR标记特异性和灵敏度检测，表明该特异引物可用于球毛壳ND35的快速检测。
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| 关 键 词： | 球毛壳ND35 RAPD SCAR标记 |
Specific RAPD- DNA Marker Transfer of Bio- control Fungus Chaetomium globosum ND35 |
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| DAI Yang,WEN Li-wei,GAO Ke-xiang,HE Bang-ling,WU Hai-yan,LIU Chang.Specific RAPD- DNA Marker Transfer of Bio- control Fungus Chaetomium globosum ND35[J].Shandong Agricultural Sciences,2011(10):8-11. |
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| Authors: | DAI Yang WEN Li-wei GAO Ke-xiang HE Bang-ling WU Hai-yan LIU Chang |
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| Institution: | (College of Plant Protection,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | This paper focused on achieving random amplified polymorphic DNA of 20 fungus isolates including Chaetomium globosum ND35,and transferring them to sequence characterized amplified region(SCAR) markers.Based on the RAPD results of C.globosum ND35,one specific DNA fragment was achieved and recycled,purified,cloned into pEASY-T3 vector,sequenced and integrated by software Seqman,then one pair of primers(CgND35-1: GTTGCCAGCCCGAGGGGAAG,CgND35-2: GTTACCAGCCATGCCTTGAAG) were designed by software Primer 5.0.With these two primers,the SCAR marker at the length of 329 bp of C.globosum ND35 was amplified successfully by the optimized PCR conditions.It was testified that this pair of specific primers could be used for rapid determination of C.globosum ND35. |
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| Keywords: | Chaetomium globosum ND35 RAPD SCAR marker |
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