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枳、富民枳与枳橙RAPD分析与分类(亲缘)关系探讨
引用本文:吴兴恩,范眸天,高峻,李文祥,龙雯虹,许明辉.枳、富民枳与枳橙RAPD分析与分类(亲缘)关系探讨[J].云南农业大学学报,2003,18(3):277-280.
作者姓名:吴兴恩  范眸天  高峻  李文祥  龙雯虹  许明辉
作者单位:[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南省农业科学院生物技术研究所,云南昆明650223
基金项目:云南省教委应用基础研究基金项目(0012033)
摘    要: 对枳、富民枳与枳橙进行了RAPD分析。17个引物对3个材料PCR扩增得52条谱带,从相似系数、多态性比较和聚类结果看:各材料之间具有不同的遗传距离,供试材料聚为2个类群(富民枳和枳橙聚为一群,枳单独聚为一类)。从分类(亲缘)关系看:作者认为富民枳与枳橙的亲缘关系更近,而与枳的关系较远。

关 键 词:  富民枳  枳橙  RAPD  分类关系
文章编号:1004-390X(2003)03-0277-04
收稿时间:2002-10-29

Studies on the RAPD Analysis and Taxonomic Relationship of Trifoliate Orange, Fuming Trifoliate Orange and Citrange
WU Xing-en,FAN Mou-tian,GAO Jun,LI Wen-xiang,LONG Wen-hong,XU Ming-hui.Studies on the RAPD Analysis and Taxonomic Relationship of Trifoliate Orange, Fuming Trifoliate Orange and Citrange[J].Journal of Yunnan Agricultural University,2003,18(3):277-280.
Authors:WU Xing-en  FAN Mou-tian  GAO Jun  LI Wen-xiang  LONG Wen-hong  XU Ming-hui
Institution:(1.College of Landscape and Horticulture, Y A U, Kunming 650201, China;2.Biotechnology Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223,China)
Abstract:RAPD analysis was applied on three species of citrus (Trifoliate orange, Fuming Trifoliate orange and Citrange). A total of 52 bands were amplified by 17 primers through PCR. The results from the similar coefficients, polymorphic comparison and clustering analysis showed that there were different genetic distances between them. Fuming Trifoliate orange and Citrange were clustered in one group and the taxonomic relationship was closer between them. Whereas Trifoliate orange was in another group and the taxonomic relationship was farther between Fuming Trifoliate orange and Trifoliate orange.
Keywords:Trifoliate orange  Fuming Trfoliate orange  Citrange  RAPD  taxonomic relationship
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