| 云南茶树DNA提取和RAPD分子标记初探 |
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| 引用本文： | 高峻,蔡新,许明辉.云南茶树DNA提取和RAPD分子标记初探[J].云南农业大学学报,2000,15(2):126-128. |
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| 作者姓名： | 高峻 蔡新 许明辉 |
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| 作者单位： | 云南农业大学农业科学技术学院,昆明 |
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| 摘 要： | 以宜良宝洪茶、莽水大叶原头茶、波上金苔、云抗10号4个云南茶树品种为材料，对其DNA提取和RAPD分子标记方法进行了研究。结果表明：所采用的经改进的CTAB DNA微量提取法，可以得到高质量的茶树DNA，分子量大于21 kb，可满足RAPD扩增；用18个不同的随机引物对所提取的4个品种基因组DNA进行了RAPD分子标记分析，其中3个（占16.67%）引物在茶树品种间可扩增出多态性产物。建立了云南茶树DNA微量、快速提取和RAPD标记的分析程序，为RAPD分析应用于云南茶树遗传研究打下了良好的基础。
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| 关 键 词： | 茶树 DNA 提取 RAPD |
| 收稿时间： | 1999-12-5 |
Tea Plant [Camellia sinensis (L.) O.Kuntze]from Yunnan |
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| Gao Jun,Cai Xin,Xu Minghui.Tea Plant [Camellia sinensis (L.) O.Kuntze]from Yunnan[J].Journal of Yunnan Agricultural University,2000,15(2):126-128. |
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| Authors: | Gao Jun Cai Xin Xu Minghui |
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| Institution: | (Faculty of Agricultural Science and Technology，Y A U，Kunming 650201) |
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| Abstract: | In this paper, a method of isolating DNA and RAPD was studied by 4 tea cultivars from Yunnan. The results showed that the good quality DNA of tea plant was gained by the isolating method and the length of DNA fragment was more than 21 kb. The isolated DNA could be used for RAPD analyses; RAPD markers with 18 random primers examined 4 tea cultivars from Yunnan, and 3 of 18 primers reveal polymorphisms among tea cultivars. This RAPD analysis procedure in this experiment lays a good foundation for application of RAPD in tea plant genetic research in Yunnan. |
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| Keywords: | Tea plant DNA isolation RAPD |
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