| HPLC-RID测定咽炎清片中左旋龙脑含量 |
| |
| 引用本文： | 赵俊杰,钱一鑫,康冀川,何珺.HPLC-RID测定咽炎清片中左旋龙脑含量[J].贵州大学学报(农业与生物科学版),2017(2):74-77. |
| |
| 作者姓名： | 赵俊杰 钱一鑫 康冀川 何珺 |
| |
| 作者单位： | 1. 贵州大学教育部西南药用生物资源工程研究中心,贵州 贵阳550025;贵州大学药学院,贵州贵阳550025;2. 贵州大学教育部西南药用生物资源工程研究中心,贵州 贵阳,550025 |
| |
| 基金项目： | 贵州省科技合作计划项目(黔科合HL字[2015]7672号)，(黔科合LH字[2016]7444号)，贵大校创字(2016006) |
| |
| 摘 要： | 以左旋龙脑标准品为对照,应用高效液相色谱法(High Performance Liquid Chromatography HPLC)(条件:Neptune C-18色谱柱,甲醇-0.4%磷酸(65∶35)为流动相,流速0.8 m L/min,柱温25℃)对咽炎清片中左旋龙脑的含量进行测定,结果发现左旋龙脑在10~100μg/m L范围内呈现良好的线性关系,其回归方程为Y=136.87X-211.73(r=0.9997,n=6),加样回收率为103.3%,RSD为0.42%,同时该方法操作简便,分析快速,方法学考察结果符合有关新药研究的技术要求及《中国药典》2015年版第四部通则中对于高效液相色谱法的各项规定,适宜用于咽炎清片中左旋龙脑含量测定。
|
| 关 键 词： | 示差检测器 咽炎清 左旋龙脑 高效液相色谱 |
Determination of L-borneol Content in Yanyanqing Tablet by HPLC-RID |
| |
| ZHAO Jun-jie,QIAN Yi-xin,KANG Ji-chuan,HE Jun.Determination of L-borneol Content in Yanyanqing Tablet by HPLC-RID[J].Journal of Mountain Agriculture & Biology,2017(2):74-77. |
| |
| Authors: | ZHAO Jun-jie QIAN Yi-xin KANG Ji-chuan HE Jun |
| |
| Abstract: | The concentration of L-borneol in Yanyanqing Tablet was determined by High Performance Liquid Chromatography (HPLC) (Conditions:C-18 Neptune column,methanol-0.4％ phosphoric acid (65 ∶35) as the mobile phase,flow rate 0.8 mL/min,column temperature 25℃).The results showed that the concentration of L-borneol had a good linear relationship within the range of 10-100 μg/mL.The regression equation was Y =136.87X-211.73 (r =0.9997,n =6).The average recovery was 103.3％,while RSD was 0.42％.It is an easy and fast approach that meet the requirements for developing new drug,as well as Chinese Pharmacopoeia 2015 (the fourth volume),by methodological study.The method is suitable for determining the concentration of L-borneol in Yanyanqing Tablet. |
| |
| Keywords: | refractive index detector Yanyanqing tablet L-borneol HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
