| 荔枝DFR基因的克隆及其序列分析 |
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| 引用本文： | 赵志常,胡福初,胡桂兵,杨转英,肖靖.荔枝DFR基因的克隆及其序列分析[J].福建农业大学学报,2013(5):512-517. |
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| 作者姓名： | 赵志常 胡福初 胡桂兵 杨转英 肖靖 |
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| 作者单位： | [1]中国热带农业科学院热带作物品种资源研究所／农业部华南作物基因资源与种质创制重点开发实验室,海南儋州571737 [2]华南农业大学园艺学院,广东广州510642 [3]海南省农业科学院热带果树研究所,海南海口571100 |
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| 基金项目： | 国家自然科学基金资助项目（30971985）;国家科技支撑计划项目（2006BAD01A1705）;现代农业产业技术体系建设专项资金资助项目（nyeytx-32）. |
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| 摘 要： | 采用同源克隆的方法从荔枝果皮中克隆得到一个二氢黄酮醇4-还原酶（DFR）基因，该基因开放阅读框全长1062bp，编码353个氨基酸．基因组扩增得到长度为2321 bp的片段，分析发现：该基因含有5个内含子，分别在119-255、426-1158、1353-1470、1632-1819、2013-2095bp之间．通过系统发育分析发现，该基因编码的蛋白与山葡萄、海棠和山竹子等果树具有较近的亲缘关系．
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| 关 键 词： | 荔枝 二氢黄酮醇4-还原酶（DFR）基因 基因克隆 序列分析 |
Cloning and sequence analysis of DFR gene from litchi |
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| ZHAO Zhi-chang,HU Fu-chu,HU Gui-bing,YANG Zhuan-ying,XIAO Jing.Cloning and sequence analysis of DFR gene from litchi[J].Journal of Fujian Agricultural University,2013(5):512-517. |
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| Authors: | ZHAO Zhi-chang HU Fu-chu HU Gui-bing YANG Zhuan-ying XIAO Jing |
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| Institution: | 1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Danzhou, Hainan 571737, China; 2. College of Horticulture, South China Agricultural University, Guangzhou, Guangdong 510642, China; 3. Institute of Tropic Fruit Trees, Hainan Academy of Agricultural Science, Haikou, Hainan 571100, China) |
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| Abstract: | The dihydroflavonol 4-reduetase （DFR） was a key enzyme in the anthocyanin biosynthesis. Degenerate primers designed according the conservative domain of dihydroflavonol 4-reductase （DFR） in plants were used to amplify the DFR fragments from the cDNA of litchi （Litchi chinensis Sonn. ）. A full length sequence of DFR gene was cloned from litchi pericarp by RACE （rapid-amplification of eDNA ends） method. The open reading frame of lltchi DFR gene was 1062 bp, and encoding 353 amino acids. The 2321 hp length fragment was amplified from genome of litchi, which contained five introns respectively, in 119 -255,426 -1158, 1353 - 1470, 1632 - 1819, 2013 -2095 bp. It was found that the amino acid sequence of litchi DFR gene had closer relationship with mountain grape （Vitis amurertsis）, begonia （Ma/us hybrid cultivar） and mangosteen （Garcinia mangostana） through phylogenetic analysis. |
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| Keywords: | litchi dihydroflavonol 4-reductase （DFR） gene gene cloning sequence analysis |
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