贵州省加工型辣椒资源RSAP分析

利用RSAP标记技术对贵州省的20份加工型辣椒种质资源进行评价,以期为辣椒品种选育提供依据。结果表明,3对引物共扩增出72条带,其中47条为多态性条带,多态性比例为65.3%,平均每对引物产生24条多态性条带,片段大小为100～2 000bp。聚类分析结果表明,20份材料的遗传相似系数变化范围为0.625～0.931,果形性状在聚类图中呈随机分布,表明贵州省加工型辣椒种质间存在着丰富的遗传多样性。

The RSAP Analysis on Processing Pepper Resources in Guizhou Province

Twenty varieties of processing pepper in Guizhou province were identified by RSAP markers,which provided evidence for breeding of processing pepper and increased stability of product quality in production enterprises.Three pairs of RSAP primers were screened and they produced 72 amplifed fragments,47 of which(65.3%) were polymorphic,with an average of 24 polymorphic fragments for each primer-pair and size ranging from 100 bp to 2 000 bp.The value of genetic similarity among 20 pepper accessions based on UPGMA varied from 0.625 to 0.931.The characters with different fruit shapes randomly distributed in the dendrogram,indicating that the genetic diversity of processing pepper cultivars from Guizhou province were abundant.