蝴蝶兰组培快繁技术研究

通过组织培养的方法诱导蝴蝶兰花梗上的腋芽萌发,可以得到无菌的蝴蝶兰不定芽。以不定芽上的幼叶为外植体,建立蝴蝶兰的无菌培养体系。结果表明:诱导腋芽萌发的培养基为MS+5 mg/L 6-BA;利用幼叶进行原球茎诱导的最佳培养基为MS+5 mg/L 6-BA+0.5 mg/L NAA+0.1 g/L活性炭(AC)+150 mL/L椰汁(CM);原球茎增殖的培养基为3 g/L花宝一号(HyponexNo.1)+5 mg/L 6-BA+1 mg/L NAA+150 mL/L CM;生根培养基为3 g/L Hyponex No.1+0.1 mg/L6-BA+1 mg/L NAA+0.5 g/L AC+100 mL/L土豆汁。

Rapid Propagation of Phalaenopsis Orchid by Tissue Culture

The axenic adventitious buds were gained by inducing the germination of the axillary bud of floral stalk in Phalaenopsis orchid.The young leaves of the axenic adventitious bud were employed as explants to establish the axenic culture system.The results showed that in MS medium with 6-BA 5mg/L,the axillary buds of floral stalk could be induced to germinate.Using the young leaves to induce the protocorm-like body,the medium with MS 5mg/L 6-BA 0.5mg/L NAA 0.1g/L AC 150mL/L coconut milk(CM)showed the best effects.The suitable media propagating protocorm-like bodies were 3g/L Hyponex No.1 5mg/L 6-BA 1mg/L NAA 150mL/L CM.In rooting stage,3g/L Hyponex No.1 0.1mg/L 6-BA 1mg/L NAA 0.5g/L AC 100mL/L potato juice was efficient.