| 油菜抗逆基因CBF1植物表达载体的构建及对拟南芥的转化 |
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| 引用本文： | 段红英,丁笑生.油菜抗逆基因CBF1植物表达载体的构建及对拟南芥的转化[J].河南农业科学,2007(11):43-46. |
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| 作者姓名： | 段红英 丁笑生 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省新乡市科技攻关项目
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河南师范大学基金 |
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| 摘 要： | 通过RT-PCR技术从油菜中克隆到抗逆相关基因CBF1,将其成功构建到植物表达载体pCAMBIA1304,并通过农杆菌采用Floral dip法将重组表达载体转入拟南芥。经潮霉素抗性筛选和PCR检测,初步证明,油菜CBF1基因已经转入到拟南芥中。
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| 关 键 词： | 油菜 拟南芥 CBF1 植物表达载体 农杆菌 潮霉素 |
| 文章编号： | 1004-3268（2007）11-0043-04 |
| 修稿时间： | 2007-06-16 |
Construction of Rapeseed CBF1 Plant Expression Vector and Its Transformation to Arabidopsis thaliana |
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| DUAN Hong-ying,DING Xiao-sheng.Construction of Rapeseed CBF1 Plant Expression Vector and Its Transformation to Arabidopsis thaliana[J].Journal of Henan Agricultural Sciences,2007(11):43-46. |
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| Authors: | DUAN Hong-ying DING Xiao-sheng |
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| Institution: | The College of Life Science, Henan Normal University, Xinxiang 453007, China |
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| Abstract: | In this study,CBF1 gene was cloned from rapeseed by RT-PCR,then was constructed into plant expression vector pCAMBIA1304.The recombinant expression vector was transformed into Arabidopsis thaliana by means of Floral dip with Agrobacterium tumefaciens.Hygromycin screening and PCR assay confirmed that the rapeseed CBF1 gene was successfully transformed into Arabidopsis thaliana. |
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| Keywords: | Rapeseed Arabidopsis thaliana CBF1 Plant expression vector Agrobacterium tumefaciens Hygromycin |
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