| 高效液相色谱法测定碧螺春茶叶中的咖啡因含量 |
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| 引用本文： | 刘臣,唐长波,姚丽萍,张雷,李邦玉.高效液相色谱法测定碧螺春茶叶中的咖啡因含量[J].江苏农业学报,2012,28(4):877-879. |
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| 作者姓名： | 刘臣 唐长波 姚丽萍 张雷 李邦玉 |
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| 作者单位： | 1. 苏州市职业大学,江苏苏州,215104
2. 苏州市出入境检验检疫局,江苏苏州,215104 |
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| 基金项目： | 2010年江苏省大学生实践创新训练项目 |
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| 摘 要： | 为了建立高效液相色谱(HPLC)法快速测定碧螺春茶叶中的咖啡因的含量,采用Shimpak C18(4.6 mm×150.0 mm)的色谱柱,以甲醇-乙腈-0.1%甲酸(20∶10∶70,体积比)为流动相,流速为1.00 ml/min,柱温为30℃,检测波长274 nm。茶叶样品中咖啡因进样量为0.275～1.650μg时咖啡因进样量(x)与峰面积(Y)呈良好的线性关系,线性回归方程为Y=1 043.500x+47.063,r2=0.999 6;平均回收率为99.68%,相对标准偏差RSD为0.23%。该方法简单、快速、准确。
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| 关 键 词： | 高效液相色谱 碧螺春茶叶 咖啡因 |
Determination of caffeine content in Biluochun tea by HPLC |
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| LIU Chen
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TANG Chang-bo
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YAO Li-ping
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ZHANG Lei
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LI Bang-yu.Determination of caffeine content in Biluochun tea by HPLC[J].Jiangsu Journal of Agricultural Sciences,2012,28(4):877-879. |
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| Authors: | LIU Chen TANG Chang-bo YAO Li-ping ZHANG Lei LI Bang-yu |
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| Institution: | 1(1.Suzhou Vocational University,Suzhou 215104,China;2.Suzhou Entry-exit Inspection and Quarantine Bureau,Suzhou 215104,China) |
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| Abstract: | To establish a rapid method for the determination of caffeine in Biluochun tea by HPLC,Shimpak C18 column(4.6 mm × 150.0 mm) was used with the mobile phase of methanol-acetonitrile-0.1% formic acid(20∶10∶70).The flowrate was 1.0 ml/min.The UV-detection wavelength was 274 nm,and column temperature was 30 ℃.The injection volume of caffeine in tea samples in the range of 0.275-1.650 μg showed a good linear relationship with the peak area and the regression equation was Y =1 043.500x + 47.063,r2=0.999 6.The average recovery was 99.68 % and the relative standard deviation(RSD) was 0.23%.The developed method was simple,rapid and accurate,and can be applied for the determination of caffeine in green tea from different areas. |
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| Keywords: | HPLC Biluochun tea caffeine |
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