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| 茉莉离体微繁及无糖生根技术 | |
| 引用本文： | 蔡汉,陈晓强,熊作明,谢玲玲,赵梁军.茉莉离体微繁及无糖生根技术[J].江苏农业学报,2007,23(5):464-468. |
| 作者姓名： | 蔡汉 陈晓强 熊作明 谢玲玲 赵梁军 |
| 作者单位： | 1. 中国农业大学观赏园艺与园林系,北京,100094;扬州大学观赏园艺系,江苏,扬州,225009 2. 江苏省农业科学院园艺研究所,江苏,南京,210014 3. 扬州大学观赏园艺系,江苏,扬州,225009 4. 中国农业大学观赏园艺与园林系,北京,100094 |
| 基金项目： | 扬州大学校科研和教改项目 , 国家科技攻关项目 |
| 摘 要： | 为解决茉莉长期无性繁殖导致的种性退化、抗逆性下降、花朵产量逐年下降等问题,进行了茉莉离体微繁及无糖生根技术的研究.结果表明:茉莉腋芽萌发的适宜培养基为MS 6-BA 2.0 mg/L NAA 0.1 mg/L;继代增殖的适宜培养基为MS 6-BA 1.5 mg/L NAA 0.3 mg/L GA3 0.5 mg/L;无糖生根的适宜培养基为1/2 MS NAA 1.2 mg/L;适宜培养条件为光强5 000～5 500 lx、CO2浓度1 500 mg/L、通气孔数为3个.无糖试管苗移栽后长势健壮,成活率可达95%. |
| 关 键 词： | 茉莉 离体微繁 无糖生根 试管苗 茉莉 离体 微繁 无糖 生根技术 Jasmine Rooting Micropropagation in vitro 成活率 试管苗移栽 气孔数 浓度 光强 培养条件 继代增殖 培养基 腋芽萌发 结果 研究 |
| 文章编号： | 1000-4440（2007）05-0464-05 |
| 修稿时间： | 2007-01-15 |
Techniques of in vitro Micropropagation and Sugar-free Rooting of Jasmine (Jasminum sambac) |
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| CAI Han,CHEN Xiao-qiang,XIONG Zuo-ming,XIE Ling-ling,ZHAO Liang-jun.Techniques of in vitro Micropropagation and Sugar-free Rooting of Jasmine (Jasminum sambac)[J].Jiangsu Journal of Agricultural Sciences,2007,23(5):464-468. | |
| Authors: | CAI Han CHEN Xiao-qiang XIONG Zuo-ming XIE Ling-ling ZHAO Liang-jun |
| Institution: | 1. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beifing 100094, China; 2. Department of Ornamental Horticulture, Yangzhou University, Yangzhou 225009, China ; 3. Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanfing 210014, China |
| Abstract: | Due to low seed-setting rate in the nature,cuttage is widely used for the reproduction of jasmine.However,long term cuttage causes varietal degeneration,resistance weakness,and declining of flower produciton.Tissue culture and micropropagation is an effective way to avoid this.The optimum media and condition for tissue culture and micropropagation of jasmine were developed.The media favoring axillary bud germination,axillary bud reproduction and sugar-free rooting were MS with the combination of BA(2.0 mg/L) and NAA(0.1 mg/L),MS with the combination of BA(1.5 mg/L),NAA(0.3 mg/L) and GA3(0.5 mg/L),and 1/2 MS with NAA(1.2 mg/L),respectively.The condition suitable for test-tube plantlet growth were light density 5 000-5 500 lx,CO2 concentration of 1 500 mg/L,and three ventholes.The sugar-free cultured plantlet grew strongly,the survival rate reaching 95%. |
| Keywords: | jasmine in vitro micropropagation sugar-free rooting test-tube plantlet |
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