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| 耐三唑类杀菌剂木霉突变体TUV-13遗传稳定性的研究 | |
| 引用本文： | 安哲宇,梁志怀,魏林,罗秀娟,陈玉荣.耐三唑类杀菌剂木霉突变体TUV-13遗传稳定性的研究[J].湖南农业科学,2010(5). |
| 作者姓名： | 安哲宇 梁志怀 魏林 罗秀娟 陈玉荣 |
| 作者单位： | 1. 中南大学研究生院隆平分院,湖南,长沙,410125 2. 湖南省植物保护研究所,湖南,长沙,410125 3. 中南大学研究生院隆平分院,湖南,长沙,410125;湖南省植物保护研究所,湖南,长沙,410125 |
| 基金项目： | 湖南省博士后科研资助专项计划项目，湖南省农村科技计划项目，国家科技支撑计划 |
| 摘 要： | 应用紫外诱变和含药培养基诱导相结合的方法,获得了一株对三唑类杀菌剂有较好耐药性的哈茨木霉突变体TUV-13.分别测定该突变体继代培养的5代,10代和20代对腈菌唑耐受能力和对病原菌的拮抗性,结果表明:该突变体的抗药性可以稳定遗传,且对病原菌的拮抗性也未丧失.同时,应用RAPD分子技术分析了不同世代菌株间的相似性. |
| 关 键 词： | 木霉 腈菌唑 耐药性 水稻病害 |
Genetic Stability of Trichoderma harzianum Mutant TUV-13 with Resistance to Triazole Fungicide |
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| AN Zhe-yu,LIANG Zhi-huai,WEI Lin,LUO Xiu-juan,CHEN Yu-rong.Genetic Stability of Trichoderma harzianum Mutant TUV-13 with Resistance to Triazole Fungicide[J].Hunan Agricultural Sciences,2010(5). | |
| Authors: | AN Zhe-yu LIANG Zhi-huai WEI Lin LUO Xiu-juan CHEN Yu-rong |
| Abstract: | Using combined method of UV-induced mutage nesis and fungicide-containing medium to obtain a Trichoderma harzianum mutant TUV-13 with strong resistance to triazole fungicide.The resistant ability and the antagonistic ability of subcultural 5th generation.10th generation and 20th generation of TUV-13 to myclobutanil and pathogen were determined respectively.The results showed that the fungicide resistance of TUV-13 could be stably inherited,and the antagonistic ability to pathogen had not Iost.Then,the similarity among different generation strains was analyzed by RAPD molecular technology. |
| Keywords: | RAFD Trichoderma harzianum myclobutanil fungicide resistance RAPD rice disease |
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