| 不同年份采收的豇豆种子的RAPD研究 |
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| 引用本文： | 乔爱民,傅家瑞.不同年份采收的豇豆种子的RAPD研究[J].仲恺农业技术学院学报,1999,12(1):26-29. |
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| 作者姓名： | 乔爱民 傅家瑞 |
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| 作者单位： | 仲恺农业技术学院园艺系(乔爱民)，中山大学生物科学与技术系(傅家瑞)，广州市农业科学研究所(吴九根) |
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| 基金项目： | 广东省博士后基金,广东省自然科学基金 |
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| 摘 要： | ＲＡＰＤ是一种建立在ＰＣＲ基础之上的新的分子标记技术，利用ＲＡＰＤ技术对不同年份采收的豇豆种子进行了研究从豇豆干种子中直接提取的基因组ＤＮＡ可以用于ＲＡＰＤ分析在２０种１０ｂｐ随机引物中筛选出Ｓ２０７和Ｓ２０８２种引物，并获得了豇豆基因组ＤＮＡ的指纹图谱筛选出的２种引物均只在１９９７年采收的豇豆种子的基因组ＤＮＡ上扩增出１条ＤＮＡ带研究结果表明，不同年份采收的豇豆种子在遗传性上相当一致，而豇豆种子的基因组ＤＮＡ在贮藏过程中会受到损伤
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| 关 键 词： | 豇豆种子，基因组DNA，RAPD |
RAPD ANALYSIS ON ASPARAGUS BEAN SEEDSHARVESTED IN DIFFERENT YEARS |
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| Qiao,AiminFu JiaruiWu Jiugen.RAPD ANALYSIS ON ASPARAGUS BEAN SEEDSHARVESTED IN DIFFERENT YEARS[J].Journal of Zhongkai Agrotechnical College,1999,12(1):26-29. |
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| Authors: | Qiao AiminFu JiaruiWu Jiugen |
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| Institution: | Qiao
Aimin1Fu Jiarui2Wu Jiugen3 |
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| Abstract: | Random amplified polymorphic DNA (RAPD) is a newly
developed molecular marker technology based on PCR (polymerase chain reaction). In this
paper, asparagus bean seeds harvested in different years were studied with RAPD. Genomic
DNA isolated directly from dry asparagus bean seeds could be used in RAPD analysis. S207
and S208 were screened from twenty 10 bp arbitrary primers, and genomic DNA fingerprints of
asparagus bean seeds were obtained. Only one DNA band was amplified on genomic DNA of
asparagus bean seeds harvested in 1997 by each of the two primers. The results showed that
asparagus bean seeds harvested in different years were genetic identical, and genomic DNA of
asparagus bean seeds might be damaged during storage. |
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| Keywords: | asparagus bean seeds genomic DNA RAPD |
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