| 反相HPLC法测定杨梅和黄皮果肉中的氨基酸 |
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| 引用本文： | 戴宏芬,赖志勇,黄炳雄,李建光,肖维强,王晓容.反相HPLC法测定杨梅和黄皮果肉中的氨基酸[J].仲恺农业技术学院学报,2008,21(3):7-11. |
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| 作者姓名： | 戴宏芬 赖志勇 黄炳雄 李建光 肖维强 王晓容 |
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| 作者单位： | 1. 广东省农业科学院果树研究所,广东,广州,510640
2. 普宁市农业科学研究所,广东,普宁,515344 |
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| 基金项目： | 广东省科技计划农业攻关计划 |
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| 摘 要： | 应用反相HPLC法对杨梅和黄皮果肉中的氨基酸进行了测定．结果表明，从杨梅和黄皮果肉中分别检测出天门冬氨酸、谷氨酸、丝氨酸、精氨酸、甘氨酸、苏氨酸、脯氨酸、丙氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸、赖氨酸和酪氨酸等14种氨基酸．其中每1g杨梅鲜果肉中的氨基酸含量为0．0379～1．7326mg；每1g黄皮鲜果肉中的氨基酸含量为0．0050～3．7692mg．两类水果不同品种之间的氨基酸含量在0．05水平上差异显著．
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| 关 键 词： | 反相HPLC 杨梅 黄皮 氨基酸 |
Determination of amino acids in flesh of Waxberry and Wampee by RP-HPLC |
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| DAI Hong-fen,LAI Zhi-yong,HUANG Bing-xiong,LI Jian-guang,XIAO Wei-qiang,WANG Xiao-rong.Determination of amino acids in flesh of Waxberry and Wampee by RP-HPLC[J].Journal of Zhongkai Agrotechnical College,2008,21(3):7-11. |
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| Authors: | DAI Hong-fen LAI Zhi-yong HUANG Bing-xiong LI Jian-guang XIAO Wei-qiang WANG Xiao-rong |
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| Institution: | DAI Hong-fen, LAI Zhi-yong, HUANG Bmg-xiong , LI Jian-guang, XIAO Wei-qiang , WANG Xiao-rong(1. Institute of Pomology, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Puning Institute of Agricultural Sciences, Puning 515344, China) |
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| Abstract: | A reversed-phase high performance liquid chromatography(RP-HPLC) method was developed for determining amino acids in flesh of waxberry and wampee.Fourteen amino acids,including Asp,Glu,Ser,Arg,Gly,Thr,Pro,Ala,Val,Ile,Leu,Phe,Lys and Tyr,were detected in samples from four waxberry and four wampee varieties.The results showed that amino acids contents ranged from 0.037 9 to 1.732 6 mg/g in fresh of waxberry and from 0.037 9 to 1.732 6 mg/g in flesh of wampee.It was indicated that amino acid contents were significantly different at 0.05 level in four varieties of each fruit. |
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| Keywords: | RP-HPLC waxberry wampee amino acid |
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