芥菜遗传多样性的RAPD分析

随机扩增多态性ＤＮＡ（ＲＡＰＤ）是一种新的分子标记技术，利用ＲＡＰＤ标记对芥菜（Ｂｒａｓｓｉｃａ ｊｕｎｃｅａ Ｃｏｓｓａ）１６个变种的遗传多样性进行了分析，从６０个１０ｂｐ随机引５物中筛选出２７个有效的，这２７个有效引物共扩增出３３６条ＤＮＡ带，其中２７５条为多态性带，占总数的８１．８５％，平均每个引物扩增出的ＤＮＡ带数为１２．４４，不同引物扩增出各自不同的ＤＮＡ指纹图谱，大部分图谱均有特征或特

RAPD analysis on genetic diversity of mustard

Random amplified polymorphic DNA (RAPD) is a newly developed molecular marker technology. In this paper, RAPD markers were applied for analysis of the genetic variations among 16 mustard (Brassica juncea Coss.) varieties. 27 10 bp arbitrary primers were screened from 60. 336 DNA bands were amplified by the 27 primers, and among them, 275 were polymorphic ones, which accounted to 81.85% of the total. The average DNA bands amplified by each primer was 12.44. Genomic DNA fingerprints amplified by different primers were obtained. Most fingerprints had their characteristic bands or band patterns. The polymorphic percentage and the average DNA bands amplified by each primer were much higher than those of most studied species, which could be considered as the molecular foundation of rich genetic diversity in mustard.