| 番茄抗晚疫病基因ph-3的RAPD及CAPS标记 |
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| 引用本文： | 吴媛媛,李海涛,张子君,邹庆道,吕书文,杨国栋.番茄抗晚疫病基因ph-3的RAPD及CAPS标记[J].沈阳农业大学学报,2009,40(6). |
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| 作者姓名： | 吴媛媛 李海涛 张子君 邹庆道 吕书文 杨国栋 |
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| 作者单位： | 1. 沈阳农业大学,园艺学院,沈阳,110161;辽宁省农业科学院,蔬菜研究所,沈阳,110161;辽宁省农业科学院,经济作物研究所,辽宁,辽阳,111000
2. 辽宁省农业科学院,蔬菜研究所,沈阳,110161 |
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| 基金项目： | 国家留学基金委资助项目，辽宁省自然科学基金项目 |
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| 摘 要： | 将番茄抗晚疫病ph-3基因已有的RAPD特异片段进行克隆、测序.根据该测序结果设计SCAR引物对抗病亲本、感病亲本、抗病池、感病池、F_1个体进行扩增,均获得一条592bp的特异片段.感病基因型和杂合抗病基因型存在Xba Ⅰ酶切位点,酶切后分别产生了261bp、193bp和95bp以及592bp、261bp、193bp和95bp的特异性片段,纯合抗病基因型无此酶切位点,酶切结果仍为592bp的产物.这些片段能成功区分抗病材料、感病材料和F_1个体,很有可能是与ph-3基因连锁的CAPS标记.
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| 关 键 词： | 番茄 晚疫病 ph-3基因 CAPS标记 |
RAPD Marker Converting to CAPS Marker of ph-3 Gene Resistant to Phytophthora infestans |
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| WU Yuan-yuan,LI Hai-tao,ZHANG Zi-jun,ZOU Qing-dao,L Shu-wen,YANG Guo-dong.RAPD Marker Converting to CAPS Marker of ph-3 Gene Resistant to Phytophthora infestans[J].Journal of Shenyang Agricultural University,2009,40(6). |
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| Authors: | WU Yuan-yuan LI Hai-tao ZHANG Zi-jun ZOU Qing-dao L Shu-wen YANG Guo-dong |
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| Institution: | WU Yuan-yuan,LI Hai-tao,ZHANG Zi-jun,ZOU Qing-dao,L(U) Shu-wen,YANG Guo-dong |
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| Abstract: | A RAPD fragment linking to ph-3 gene was cloned. According to the sequencing result,we designed SCAR primer. A 592bp fragment was amplified in resistant and susceptible parents, resistant and susceptible bulks, F_1 individuals,and then the PCR products were digested with restriction enzyme XbaI. Susceptible genotypes and heterozygous resistant genotypes could produce respectively 261bp,193bp,95bp and 592bp,261bp,193bp,95bp bands.Homozygous resistant genotypes presented 592bp fragment.These DNA bands which could distinguish resistant material,susceptible material and F_1 individuals successfully, were probably CAPS markers for ph-3 gene. |
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| Keywords: | tomato late blight gene Ph-3 CAPS maker |
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