利用分子标记对桃属植物的识别及其亲缘关系分析

采用RAPD技术，用筛选的22个随机引物对桃属主要种类及其类型进行DNA扩增分析。结果表明不同引物扩增出的DNA片段范围在3～13条，分子量范围在300bp～3kb之间，依据特征谱带，可建立种间及其类型识别的分子检索表。利用22个引物扩出的180个位点上的RAPD带，进行聚类分析，结合前人研究出的结果，揭示桃种间亲缘演化顺序为内蒙古长柄扁桃→光核桃→山桃→甘肃桃→白花山桃、红花山桃、帚形山桃→桃巴旦、陕西桃巴旦→新疆桃、毛桃。

The Identification and Systematic Relationship Analysis of Amyg dalus spp. by Molecular Markers

Total DNAs of major species and types in Amygda lus L. were amplified with selected 22 arbitrary primers by using RAPD.The results showed that the nu m ber of DNA fragments amplified by different primers varied from 3 to 13,molecula r sizes from 300 bp to 3 kb.The tested accessions could be identified by the amplified bands. According to special bands, a chart capable of distinguishing the species and types was figured out. The cluster a nd anal ysis of RAPD bands in 180 loci of 22 arbritrary primers, involving the relative research results, revealed the systematic evolution of the experimented sp ecies as follow:Neimenggu changbinbiantao(A. pedunculata)→Guanghetao(A.mi ra)→ Shantao(A.davidiana)→Gansutao(A.kansuensis)→Baihuashantao，Honghuash antao，Zou xingshantao(A.davidiana)→Taobadan,Shanxi taobadan(A.communis)→Xinjiang tao(A.ferganensis)，Maotao(A.persica).