氰戊菊酯对鲤SOD活性和MDA含量以及肝肾细胞形态的影响

通过不同质量浓度的氰戊菊酯对鲤进行21d半静置染毒,测定不同时间血清、肝脏组织中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化,观察染毒后氰戊菊酯对鲤肝、肾组织结构的影响。结果表明:暴露于氰戊菊酯中的鲤,肝脏及血清中SOD活性均呈现先上升后降低的变化规律,且血清中SOD活性变化先于肝脏中的变化,暴露21d后活性极显著低于空白对照组;而MDA含量均随着暴露时间的延长显著增加,上升时间主要集中在暴露后的第3～14天。苏木精-伊红染色显示,鲤肝血窦扩张充血,肝细胞空泡变性及溶解并形成局部坏死病灶等,0.8μg/L染毒第21天、4.05μg/L和20.25μg/L染毒3d后上述损伤明显;鲤肾间质细胞增多,炎性细胞浸润,部分肾小管与基底膜分离,管壁细胞水样变性,高剂量组染毒21d还兼有肾小球萎缩,大量含铁血黄素沉着等。超显微观察发现,高剂量染毒21d肝细胞核萎缩,核内异形染色体边集化、粗面内质网水肿、松散甚至断裂,胞质中出现空泡,脂滴大量增多等。以上损伤不仅具有剂量相关性,还具有时间积累效应。

Effect of fenvalerate on activity of SOD,content of MDA and histomorphology of liver and kidney of carp

The effects of different concentration of fenvalerate on the activity of superoxide dismutase(SOD),content of malondialdehyde(MDA) in serum and liver and histomorphology of liver and kidney of common carp were studied.The results indicated that in the serum and liver,the activities of SOD in the treatment groups increased first,then decreased,and the activity of SOD in the serum incresed earlier than that in the liver.The activities of SOD in the experimental groups were all significantly lower than that in the control group after exposure in fenvalerate for 21 d(P<0.01).The content of MDA was significantly increased with the increase of exposing time,especialy between 3 and 14 d after exposure.The result of HE stain dyeing showed that in the liver,hepatic sinusoid dilated and congested,liver cell denatured and dissolved,and even worse,local necrosis appeared,which were more noticeable after exposure in 0.8 μg/L fenvalerate for 21 d and in 4.05 or 20.25 μg/L fenvalerate for 3 d.In the kidney of common carp,renal interstitial cells increased,lymphocytic cell infiltrated,some basement membrane separated from renal tubular,and renal tubular cells hydropic degenerated.The glomerulus shrunk and a large amount of hemosiderin deposited in the high concentration group after exposure for 21 d.The ultramicroscopical observation of liver cells after exposure in high concentration fenvalerate for 21 d showed that the marginal heterochromatined,the rough endoplasmic reticulum swelled,loosed and fratured and lipid droplets increased.The above damage was not only related to the concentration,but also had time accumulative effect.