| 蜡梅的离体培养与植株再生 |
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| 引用本文： | 曾静,眭顺照,余国武,雷兴华,李名扬.蜡梅的离体培养与植株再生[J].西南农业大学学报,2007,29(4):120-123. |
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| 作者姓名： | 曾静 眭顺照 余国武 雷兴华 李名扬 |
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| 作者单位： | 西南大学园艺园林学院花卉研究所,重庆400716 |
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| 基金项目： | 重庆市自然科学基金项目（CSTC,2006BB1336）;重庆市教育委员会科学研究项目（100136）. |
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| 摘 要： | 以蜡梅种子无菌苗子叶作为外植体,以改良MS和MS作为基本培养基,添加不同激素组合,筛选出诱导蜡梅愈伤组织产生和分化的最佳培养基,建立蜡梅的再生体系.试验结果表明,改良MS＋6-BA 1.0 mg/L＋NAA0.5 mg/L＋KT 0.5 mg/L＋IBA 0.2 mg/L＋2.4-D 0.2 mg/L能诱导蜡梅愈伤组织的产生和分化,再生植株在改良MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/上增殖系数最高,能达到2.7.1/2 MS＋NAA 0.1 mg/L能有效促进蜡梅生根,生根率在70%以上,而在未添加NAA的1/2 MS中生根率为10%.
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| 关 键 词： | 蜡梅 子叶 再生 增殖 离体培养 |
| 文章编号： | 1000-2642（2007）04-0120-04 |
| 修稿时间： | 2006-12-25 |
In Vitro Culture and Plant Regeneration of Chimonanthus praecox Link. |
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| ZENG Jing, SUI Shun-zhao, YU Guo-wu, LEI Xing-hua, LI Ming-yang.In Vitro Culture and Plant Regeneration of Chimonanthus praecox Link.[J].Journal of Southwest Agricultural University,2007,29(4):120-123. |
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| Authors: | ZENG Jing SUI Shun-zhao YU Guo-wu LEI Xing-hua LI Ming-yang |
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| Institution: | School of Horticulture and Landscape Architecture, Institute of Ornamental Plants, Southwest University, Chongqing 400716, China |
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| Abstract: | The cotyledons of Chimonanthus praecox were used as explants in in viteo culture.The modified MS medium and MS medium were used as the basic medium in this experiment.The results showed that the regeneration and cytodifferentiation of calli were induced in the modified MS+6-BA1.0 mg/L+NAA 0.5 mg/L+KT 0.5 mg/L+IBA 0.2 mg/L+2,4-D0.2 mg/L.The regenerated plantlets had the best growth coefficient(2.7) in the modified MS+6-BA1.0 mg/L+NAA0.1 mg/L.The optimum medium for rhizogenesis was 1/2 MS+NAA 0.1 mg/L,where the rate of rhizogenesis could be more than 70%.In contrast,the rate of rhizogenesis was only 10% in 1/2 MS without any NAA. |
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| Keywords: | Chimonanthus praecox Link cotyledon plant regeneration multiplication in vitro culture |
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