| 大花蕙兰原球茎的诱导和增殖研究 |
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| 引用本文： | 宋丽莎,乙引,张习敏,张宇斌.大花蕙兰原球茎的诱导和增殖研究[J].贵州农业科学,2010,38(1). |
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| 作者姓名： | 宋丽莎 乙引 张习敏 张宇斌 |
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| 作者单位： | 1. 贵州师范大学,生命科学学院,贵州,贵阳,550001;黔南民族师范学院,生命科学系,贵州,都匀,558000
2. 贵州师范大学,生命科学学院,贵州,贵阳,550001 |
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| 基金项目： | 贵州省科技创新人才团队建设项目“贵州省特色植物资源保护与可持续利用”[黔科合人才团队(2009)4007] |
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| 摘 要： | 通过对大花蕙兰茎尖和茎段的组织培养,建立了大花蕙兰原球茎的无菌繁殖体系。结果表明:芽诱导的最适培养基为1/2MS+0.2 mg/L NAA+1.0 mg/L 6-BA+100 mg/mL VC;原球茎诱导培养基为1/2MS+0.2 mg/L NAA+2.5 mg/L 6-BA+100 mg/mLVC;原球茎诱导增殖培养基为1/2MS+0.3 mg/L NAA+3.0 mg/L 6-BA+100 mg/mL VC;适宜原球茎增殖培养的外植体为原球茎。
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| 关 键 词： | 大花蕙兰 原球茎 培养基 |
Induction and Propagation of Hybridum cymbidium Protocorms |
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| SONG Li-sa,YI Yin,ZHANG Xi-min,ZHANG Yu-bin.Induction and Propagation of Hybridum cymbidium Protocorms[J].Guizhou Agricultural Sciences,2010,38(1). |
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| Authors: | SONG Li-sa YI Yin ZHANG Xi-min ZHANG Yu-bin |
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| Institution: | 1.School of Life Sciences;Guizhou Normal University;Guiyang;Guizhou 550001;2.Department of Life Sciences;Qiannan National Normal College;Duyun;Guizhou 558000;China |
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| Abstract: | The aseptic propagation system of Hybridum cymbidium protocorms was established by the tissue culture of its stem apex and section.The results showed that the optimum induction media for bud,and protocorm were 1/2MS+0.2mg/L NAA+1.0 mg/L 6-BA +100 mg/mL VC and 1/2MS+0.2 mg/L NAA+2.5 mg/L 6-BA +100 mg/mL VC respectively,the optimum multiplication medium for protocorm induction was 1/2MS+0.3 mg/L NAA+3.0 mg/L 6-BA +100mg/mL VC,and the suitable explant for protocorm multiplication of Hybridum cymbidium was prot... |
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| Keywords: | Hybridum Cymbidium protocorm medium |
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