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德州驴RAPD遗传多态性分析
引用本文:刘娟,焦传珍.德州驴RAPD遗传多态性分析[J].广东农业科学,2010,37(11):213-214.
作者姓名:刘娟  焦传珍
作者单位:德州学院生物系,山东,德州253023
基金项目:山东省自然科学基金(Y2007D55)
摘    要:采用RAPD技术探讨了德州驴的遗传多态性。从20条随机引物中筛选出8条引物对德州驴56个个体的基因组DNA进行随机扩增。结果表明,8条随机引物共扩增产生了784条谱带,其中多态性条带为165条,多态比率在11.1%~37.5%之间;与其他驴的遗传多样性研究结果相比,德州驴的遗传多样性处于中等水平。

关 键 词:德州驴  RAPD  遗传多态性  基因组DNA  

RAPD analysis of the genetic diversity in donkey of Dezhou
LIU Juan,JIAO Chuan-zhen.RAPD analysis of the genetic diversity in donkey of Dezhou[J].Guangdong Agricultural Sciences,2010,37(11):213-214.
Authors:LIU Juan  JIAO Chuan-zhen
Institution:LIU Juan,JIAO Chuan-zhen(Biology Department of Dezhou University,Dezhou 253023,China)
Abstract:In this study,Dezhou donkey were analyzed using RAPD technology and their genetic diversity were discussed.8 primers were chosen from 20 random primers for the amplification of the 24 individual's genomic DNA.The results showed that a total of 784 clear and reproducible bands were amplified by 8 screened random primers,and there were 165 polymorphic bands with the polymorphic rate from 11.1% to 37.5%.Comparing the genetic diversity of other donkeys,the genetic diversity in Dezhou donkey was in medium state.
Keywords:donkey of Dezhou  RAPD  genetic diversity  genomic DNA  
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