| 鸭瘟病毒河南株gI基因的克隆与测序 |
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| 引用本文： | 王岩,杨明凡,崔保安,阴晓霞,邓永欢,闫艺克,王汉邦.鸭瘟病毒河南株gI基因的克隆与测序[J].广东农业科学,2012,39(3):139-140. |
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| 作者姓名： | 王岩 杨明凡 崔保安 阴晓霞 邓永欢 闫艺克 王汉邦 |
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| 作者单位： | 1. 郑州牧业工程高等专科学校动物医学系,河南郑州,450011
2. 河南农业大学牧医工程学院,河南郑州,450002 |
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| 基金项目： | 河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 参考GenBank中鸭瘟病毒gI基因序列设计并合成引物,以鸭瘟病毒河南分离株DNA为模板进行PCR扩增,将扩增片段克隆至PGEM-T载体上,得到含gI基因重组质粒。经酶切鉴定,并对重组阳性质粒进行序列测定。结果表明,获得的片段含鸭瘟病毒gI基因,全长1 089 bp,与已报道的其他疱疹病毒gI基因具有较高的同源性,编码432个氨基酸,蛋白分子质量为39.7ku、等电点(PI)为6.06。
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| 关 键 词： | 鸭瘟病毒 gI基因 克隆 测序 |
Clone and sequencing of gI gene of duck plague virus Henan strain |
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| WANG Yan
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YANG Ming-fan
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CUI Bao-an
,
YIN Xiao-xia
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DENG Yong-huan
,
YAN Yi-ke
,
WANG Han-bang.Clone and sequencing of gI gene of duck plague virus Henan strain[J].Guangdong Agricultural Sciences,2012,39(3):139-140. |
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| Authors: | WANG Yan YANG Ming-fan CUI Bao-an YIN Xiao-xia DENG Yong-huan YAN Yi-ke WANG Han-bang |
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| Institution: | 1(1.Zhengzhou Colloge of Animal Husbandry and Veterinary,Zhengzhou 4500011,China; 2.Engineering College of Animal Husbandry and Veterinary Science,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | According to published duck plague virus(DPV)gI gene sequences,a pair of specific primers was designed and synthesized.The genomic DNA of DPV Henan strain,which was isolated from Henan,was used as template.The PCR product cloned into the PGEM-T vector.The gene was sequenced and a complete open reading frame was found by bioinformatics tools of ORF Finder and Blastn.Bioinformatics analysis showed that the ORF of 1 089 bp encoding gI gene and encoding 362 amino acids was obtained.The protein molecular weight was 39.7 ku,and its isoelectric point PI was 6.060. |
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| Keywords: | DPV gI gene cloning sequencing |
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